| 牛体外发育胚胎特定阶段差异表达基因研究 |
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| 引用本文： | 栗雪冰,仓明.牛体外发育胚胎特定阶段差异表达基因研究[J].安徽农业科学,2010,38(23):12476-12478. |
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| 作者姓名： | 栗雪冰 仓明 |
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| 作者单位： | 内蒙古大学哺乳动物生殖生物学和生物技术教育部重点实验室,内蒙古呼和浩特,010021;内蒙古大学哺乳动物生殖生物学和生物技术教育部重点实验室,内蒙古呼和浩特,010021 |
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| 摘 要： | 目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异。方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比。结果]该序列与牛核糖体蛋白L31（ribosomal protein L31,RPL31）基因序列具有99%的同源性。采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍。结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据。
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| 关 键 词： | 单胚mRNA差异显示 牛胚胎 基因 实时定量PCR |
Study on the Gene Expression of Preimplantation IVF Bovine Embryos |
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| Institution: | LI Xue-bing et al (Key Laboratory of China Education Ministry for Research of Mammal Reproductive Biology and Biotechnology,Inner Mongol University ,Hohhot Inner Mongol 010021) |
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| Abstract: | Objective]The aim was to study gene expression of different stage embryos of cattle obtained from in vitro. Method] Single 8-cell and blastocyst stage embryos were studied by using technology of single preimplantation embryo mRNA different display and one different fragment was found,which was then cloned,sequenced and blaste with GenBank. Result]The results suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31. Then the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos was detected by real-time quantitative PCR,the result showed the relative amount of 8 cell was 3.2 times of blastocyst's.Conclusion]This study provided a basis for revealing and clarifing related mechanism of controling bovine early embroyonic development. |
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| Keywords: | Single preimplantation embryo mRNA different display Cattle embryo Gene Real-time quantitative PCR |
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