| 曲霉Aspergillus sojae壳聚糖酶的诱导及特性 |
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| 引用本文： | 张学昆,李加纳,陆合.曲霉Aspergillus sojae壳聚糖酶的诱导及特性[J].农业生物技术学报,2004,12(4):450-454. |
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| 作者姓名： | 张学昆 李加纳 陆合 |
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| 作者单位： | 西南农业大学农学与生命科学学院,重庆,470016 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 曲霉(Aspergillus sojae)在Czapek-Dox培养基中加入2％N-乙酰氨基葡糖、1％蛋白胨和0．5％酵母膏，26℃、150r／min摇瓶培养下，在60h诱导产生较强的壳聚糖酶活性(达12．34mU／mL)，同时形成了4种壳聚糖同工酶，CHA1、CHA2、CHA3和cHA4，蛋白质分子量约为38．2、30．8、27．7和25．0kD。对诱导的壳聚糖酶部分特性初步研究表明，该酶属于壳聚糖内切酶，不能分解胶体几丁质、乙二醇几丁质、干粉几丁质和羧甲基纤维素，但能随乙酰化程度的增加有效分解壳聚糖。利用薄层层析表明，该酶可将壳聚糖分解得到的产物为壳聚二糖。
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| 关 键 词： | Aspergillus 壳聚糖酶 诱导 曲霉 蛋白质分子量 羧甲基纤维素 几丁质 乙酰氨基 摇瓶培养 薄层层析 培养基 酵母膏 蛋白胨 min 同工酶 内切酶 分解 乙二醇 乙酰化 干粉 产物 |
Induction and Some Properties of Chitosanase from Aspergillus sojae |
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| Abstract.Induction and Some Properties of Chitosanase from Aspergillus sojae[J].Journal of Agricultural Biotechnology,2004,12(4):450-454. |
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| Authors: | Abstract |
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| Abstract: | After chitosanase induction in the optimal culture conditions of Aspergillus sojae, Czapek-Dox medium (CD) +2%N-acetylglucosamine (GlcNAc)+1% peptone+0.5% yeast extract, 26℃ and 150 r/min for 60 h, the chitosanolytic activity reached a maximum level (12.34 mU/mL), and four isoenzymes were detected by active staining after SDS-PAGE, CHA1, CHA2, CHA3 and CHA4, with molecular weights of about 38.2, 30.8, 27.7 and 25.0 kD, respectively. Some properties showed the chitosanolytic enzyme was an endo-chitosanase. Analysis of reaction products by thin-layer chromatography (TLC) indicated that the chitosanase hydrolyzed chitosan 10B(D.A.0%) into chitobiose. The chitosanase cleaved chitosan 7B (D.A.30%) with the maximum velocity and the activity declined with decreasing degree of acetylation (D.A.) of chitosan-type substrates,but difficultly hydrolyzed chitin-type substrates. |
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| Keywords: | Aspergillus sojae chitosan chitosanase induction characteristic |
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