| 对引进的美国大豆品种进行转基因成分的检测 |
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| 引用本文： | 宋扬,吴存祥,侯文胜,韩天富.对引进的美国大豆品种进行转基因成分的检测[J].大豆科学,2005,24(2):116-120. |
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| 作者姓名： | 宋扬 吴存祥 侯文胜 韩天富 |
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| 作者单位： | 中国农业科学院作物科学研究所,北京,100081;中国农业科学院作物科学研究所,北京,100081;中国农业科学院作物科学研究所,北京,100081;中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 引进国际先进农业科技计划(948计划)，北京市自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | 利用田间表型鉴定和PCR检测相结合的方法,对从美国引进的46个大豆品种进行了转基因成分的检测.实验过程中,首先通过喷洒草甘膦对待测材料进行表型鉴定,然后通过PCR分子检测分析CaMV35S启动子和NOS终止子的有无,初步判定引进材料是否为转基因大豆,最后对目的基因CP4-EPSPS进行检测,进一步确定所测材料是否为抗草甘膦转基因大豆.大豆本身的凝集素基因作为PCR检测的内置检测标准,有效排除了由于DNA质量、实验操作等因素对检测结果的影响.结果表明,在所检测的46个引进品种中,5个是抗草甘膦转基因品种,41个是非转基因品种,它们可作为我国大豆新品种选育的亲本材料.
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| 关 键 词： | 转基因大豆 草甘膦 PCR CP4-EPSPS |
| 文章编号： | 1000-9841(2005)02-0116-05 |
IDENTIFICATION OF THE TRANSGENIC ELEMENTS IN THE INTRODUCED AMERICA SOYBEAN LINES |
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| Song Yang,Wu Cunxiang,Hou Wensheng,Han Tianfu.IDENTIFICATION OF THE TRANSGENIC ELEMENTS IN THE INTRODUCED AMERICA SOYBEAN LINES[J].Soybean Science,2005,24(2):116-120. |
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| Authors: | Song Yang Wu Cunxiang Hou Wensheng Han Tianfu |
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| Abstract: | In the present study, both field screening and PCR detection were conducted to identify the transgenic elements in 46 accessions of introduced America soybean varieties. First, the introduced varieties were screened by foliar spaying of glyphosate at lethal concentration in the field; Secondly, CaMV35S promoter, NOS terminator and lectin genes were examined by using PCR to determine if these lines were genetically modified; Finally, CP4-EPSPS and lectin genes were amplified with PCR to determine if they were glyphosate-resistant transgenic soybeans. The lectin gene of soybean itself was used as internal standard to exclude the interference of DNA quality and experimental operation problems. Among the forty-six introduced America soybean varieties, five were found to be glyphosate-resistant and transgenic, and other forty-one were nontransgenic. The latter can be used as parents in soybean breeding program in difference regions of China. |
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| Keywords: | PCR CP4-EPSPS |
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