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A laboratory test protocol to evaluate effects of plant protection products on mortality and reproduction of the predatory mite<Emphasis Type="Italic">hypoaspis aculeifer</Emphasis> canestrini (acari: laelapidae) in standard soil
Authors:Email author" target="_blank">Frank?M?BakkerEmail author  Rik?Feije  André?J?Grove  Gijs?Hoogendoorn  Gerrit?Jacobs  Edwin?D?Loose  Pam?van?Stratum
Institution:(1) MITOX Trial Management BV, PO BOX 92260, 1090 AG Amsterdam, The Netherlands;(2) MITOX Laboratories BV, PO BOX 92260, 1090 AG Amsterdam, The Netherlands;(3) Mitax BV, PO BOX 92260, 1090 AG Amsterdam, The Netherlands;(4) University of Leiden, PO BOX 9516, 2300 RA Leiden, The Netherlands
Abstract:We provide a test protocol to evaluate the effects of plant protection products on mortality and reproductive performance ofHypoaspis aculeifer (Acari: Laelapidae). The test design is in compliance with standard characteristics of extended laboratory studies as formulated by international expert panels dealing with non-target arthropod testing schemes. Potential effects on survival rates are evaluated by assessing mortality after an exposure period of 14 days that starts with protonymphs. The reproductive performance of surviving mites is studied by following the egg production of individually isolated females over a 7-day period. The hatching success of these eggs is assessed subsequently. Using several years’ data obtained from water control treatments, we propose criteria to evaluate the validity of individual experiments.
Keywords:Extended laboratory test  Hypoaspis aculeifer  non-targetb arthropods  plant protection products  soil  test protocol
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