| 关键因子对棉花利用农杆菌介导法导入外源基因的影响 |
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| 引用本文： | 李宝平,赵俊侠,石跃进,郭三堆,桑瑜,齐宏立,焦改丽,陈志贤.关键因子对棉花利用农杆菌介导法导入外源基因的影响[J].作物学报,2001,27(1):80-84. |
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| 作者姓名： | 李宝平 赵俊侠 石跃进 郭三堆 桑瑜 齐宏立 焦改丽 陈志贤 |
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| 作者单位： | 山西省运城高等专科学校， 山西运城 044000 |
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| 基金项目： | 本项目为国家863计划和山西省科委资助项目(DH02-01-01(Z17-01-01)) |
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| 摘 要： | 用5～6d的棉花无菌苗下胚轴切段与农杆菌共培，将Bt基因和tfdA基因导入棉花。菌株质粒中GUS基因为标记基因，NPTⅡ基因为选择基因。在含有0.1mg/L2,4-D和0.1mg/LKT的MS培养基上共培48h，转移到添加头孢霉素500mg/L、卡那霉素50mg/L培养基中诱导和筛选抗性愈伤组织。70～80d时，进行GUS检测，选择GUS阳性愈伤组织，经体细胞胚
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| 关 键 词： | 棉花 农杆菌 基因转化 再生植株 GUS基因 |
| 收稿时间： | 1999-06-21 |
| 修稿时间： | 1999年6月21日 |
Influence of Some Factors
on Transgenic Cotton by Agrobacterium-mediated Gene Transfer |
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| LI Bao-Ping,ZHAO Jun-Xia,SHI Yue-Jin,GUO San-Dui,SANG Yu,QI Hong-Li,JIAO Gai-Li,CHEN Zhi-Xian.Influence of Some Factors
on Transgenic Cotton by Agrobacterium-mediated Gene Transfer[J].Acta Agronomica Sinica,2001,27(1):80-84. |
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| Authors: | LI Bao-Ping ZHAO Jun-Xia SHI Yue-Jin GUO San-Dui SANG Yu QI Hong-Li JIAO Gai-Li CHEN Zhi-Xian |
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| Abstract: | Hypocotyl segments from 5～6 day old seedlings of Grossypiumhirsutum were cocultured with Agrobacterium tumefaciens in order to introduce Bt and tfdA genes into cotton crop. GUS gene is as marker gene and NPTⅡ gene is as selecting gene. The cultures were cocultured in MS medium contained 0.1 mg/L 2,4-D and 0.1 mg/L KT for 48h, then transfered into the medium supplemented with 500 mg/L Cefotaxime and 50 mg/L Kanamycin to induce and select resistant calli. After 70～80 days, GUS gene was inspected and the positive calli were selected and cultured on embryogenesis medium until globular embryos developing and germinated plantlets forming. |
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| Keywords: | Cotton Agrobacterium tumefaciens Gene transformation Regenerated plant GUS gene |
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