Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by a new cytometric assay |
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| Authors: | Aguedo Mario Waché Yves Mazoyer Virginie Sequeira-Le Grand Anabelle Belin Jean-Marc |
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| Institution: | Laboratoire de Microbiologie, UMR UB/INRA 1082, ENSBANA, and Centre de Cytométrie en Flux, Université de Bourgogne, 21 000 Dijon, France. |
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| Abstract: | The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is promising for various applications concerning the study of interactions between microorganisms and an emulsified hydrophobic substrates. |
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