| 抗氧化剂和吸附剂促进银杏愈伤组织生长及防止褐化作用研究 |
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| 引用本文： | 邵菊芳,朱红威,于文峰,张伟.抗氧化剂和吸附剂促进银杏愈伤组织生长及防止褐化作用研究[J].福建林业科技,2009,36(3). |
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| 作者姓名： | 邵菊芳 朱红威 于文峰 张伟 |
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| 作者单位： | 中国矿业大学化工学院,江苏徐州,221008 |
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| 基金项目： | 中国矿业大学青年科技基金，中国矿业大学大学生科研训练计划 |
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| 摘 要： | 探讨了抗氧化剂柠檬酸、抗坏血酸(VC)、植酸(PA)和聚乙烯吡咯烷酮(PVP),吸附剂活性炭(AC)对银杏愈伤组织继代培养中细胞生长和褐化的影响,结果表明:柠檬酸0.2 g.L-1对银杏继代细胞生长速率促进最大,可达11.354%;15 mg.L-1VC抗褐化效果最好,叶绿素含量可达0.248%。综合细胞生长和抗褐化效果,最佳处理为柠檬酸0.2 g.L-1。
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| 关 键 词： | 银杏 抗氧化剂 吸附剂 愈伤组织 细胞生长 细胞褐化 |
Study on Growth Promotion and Browning Prevention of Ginkgo Callus with Antioxidant and Sorbent |
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| SHAO Ju-fang,ZHU Hong-wei,YU Wen-feng,ZHANG Wei.Study on Growth Promotion and Browning Prevention of Ginkgo Callus with Antioxidant and Sorbent[J].Journal of Fujian Forestry Science and Technology,2009,36(3). |
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| Authors: | SHAO Ju-fang ZHU Hong-wei YU Wen-feng ZHANG Wei |
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| Institution: | SHAO Ju-fang,ZHU Hong-wei,YU Wen-feng,ZHANG Wei(Chemical Technology College,China Mining University,Xuzhou 221008,Jiangsu,China) |
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| Abstract: | The effects of five antioxidants and sorbent,including citric acid,VC,PA and PVP,AC,on cell growth and browning in ginkgo callus generation-continuing culture were discussed in this article.The results showed that:The growth rate,11.354%,of ginkgo generation-continuing cell was mostly influenced by 0.2 g·L-1 of citric acid;The best aiti-browning substract was 15 mg·L-1 of VC.Chlorophyl content was 0.248%.Synthetically considering the cell growth and aiti-browning effects,the best treatment was 0.2 g·L-1 of citric acid. |
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| Keywords: | ginkgo antioxidant sorbent callus cell growth cell browning |
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