| 猪伪狂犬病病毒FZ株的分离鉴定及gD基因序列分析 |
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| 引用本文： | 曾显成,吴异健,陈家祥,姚金水,吴宝成.猪伪狂犬病病毒FZ株的分离鉴定及gD基因序列分析[J].福建农林大学学报(自然科学版),2011,40(5):501-510. |
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| 作者姓名： | 曾显成 吴异健 陈家祥 姚金水 吴宝成 |
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| 作者单位： | 福建农林大学动物科学学院,福建福州,350002 |
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| 摘 要： | 采集福州郊区病仔猪的鼻腔黏液、脑、脾脏、肾脏、淋巴结等组织病料,研磨上清接种到非洲绿猴肾(Vero)细胞和狗肾(MDCK)细胞进行病毒分离,通过对分离病毒株进行形态学、血清学、荧光抗体试验、动物接种试验、PCR试验等鉴定,确定所分离的病毒为猪伪狂犬病病毒(PRV),命名为PRV-FZ株.根据GenBank收录的PRVg...
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| 关 键 词： | 猪伪狂犬病毒 分离鉴定 FZ株 gD基因 序列分析 |
Isolation and identification of porcine pseudorabies virus FZ strain and sequence analysis of the gD gene of the strain |
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| ZENG Xian-cheng,WU Yi-jian,CHEN Jia-xiang,YAO Jin-shui,WU Bao-cheng.Isolation and identification of porcine pseudorabies virus FZ strain and sequence analysis of the gD gene of the strain[J].Journal of Fujian Agricultural and Forestry University,2011,40(5):501-510. |
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| Authors: | ZENG Xian-cheng WU Yi-jian CHEN Jia-xiang YAO Jin-shui WU Bao-cheng |
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| Abstract: | A wild-type pseudorabies virus(PRV) was isolated from an affected porcine in Fuzhou.Supernatants from cell extracts of nose cavity mucus,brain,spleen,kindy,lymph were inoculated to the Vero cells and MDCK cells and significant cytopathogenic effect was produced.The isolated virus was confirmed by serological tests,PRV-Fluorescence antibody tests,experimental infection of animals and PCR tests.The virus isolate was designated as PRV-FZ.A pair of oligonucleotide primers were designed according to the sequences published by the GenBank in order to amplifiy gD gene of the PRV-FZ.A PCR product of approximately 1579 bp was amplified from PRV-FZ strain.The PCR product was inserted into PCAGGS vector and sequenced.Nucleotide sequencing revealed that this fragment contained an open reading frame of 1203 bp encoding a 400 aa protein.The gene was sequenced and compared with those of five other PRV strain in GenBank.The results showed that the gD gene of PRV-FZ shared 99.1%-99.8% nucleotide homology and 98.5%-99.5% amino acid homology with strains.Meanwhile there are two notable transmembrane region in PRV-FZ gD by Bioedit and TMPRED;Structure prediction shows that there are two CAMP_PHOSPHO_SITE,five PKC_PHOSPHO_SITE,one CK2_PHOSPHO_SITE and three pires of disulfind. |
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| Keywords: | pseudorabies virus isolation and identification FZ strain gD gene sequence analysis |
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