Genetic variability in wild populations of <Emphasis Type="Italic">Prunus divaricata</Emphasis> Ledeb. in northern Iran evaluated by EST-SSR and genomic SSR marker analysis |
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| Authors: | Tina Wöhrmann Daniela Guicking Korous Khoshbakht Kurt Weising |
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| Institution: | (1) Plant Molecular Systematics, University of Kassel, 34109 Kassel, Germany;(2) Environmental Science Research Institute, Shahid Beheshti University G. C., Tehran, Iran; |
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| Abstract: | A population genetic analysis based on eight genomic SSR markers and three EST-SSR (expressed sequence tags) markers developed
in peach (Prunus persica (L.) Batsch) and Japanese plum (Prunus salicina Lindl.) was carried out in 12 wild populations of cherry plum (Prunus divaricata Ledeb.) sampled along the Iranian coast of the Caspian Sea. A total of 184 alleles (3–31 per locus) were detected with a
mean value of 16.7 alleles per locus. None of the loci or populations showed deviation from Hardy–Weinberg equilibrium, and
all markers proved to be unlinked. The mean values for the observed and the expected heterozygosity were 0.66 and 0.73, respectively.
There was very little genetic differentiation among populations, as was indicated by low overall values of Wright’s FST (0.03) and Nei’s GST (0.08). An analysis of molecular variance (AMOVA) showed that 96.8% of the total variance was attributable to differences
between individuals within populations. Genetic and geographic distances were nevertheless positively correlated, as evidenced
by a Mantel test. The high level of genetic diversity and the apparent lack of genetic structure in wild P. divaricata may be attributed to frequent long distance gene flow through frugivorous birds and possibly humans, as has been documented
for other Prunus species. |
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