| 猪CART mRNA全CDS区序列的克隆与表达载体的构建 |
| |
| 引用本文： | 李鹏飞,李富禄,于秀菊,吕丽华.猪CART mRNA全CDS区序列的克隆与表达载体的构建[J].福建农林大学学报(自然科学版),2011,40(6):610-613. |
| |
| 作者姓名： | 李鹏飞 李富禄 于秀菊 吕丽华 |
| |
| 作者单位： | 山西农业大学生命科学学院,山西太谷,030801 |
| |
| 基金项目： | 农业部“948”项目，山西省留学基金和山西省引进人才专项基金，山西省国际科技合作项目 |
| |
| 摘 要： | 选用猪为研究对象,以可卡因苯异丙胺调控转录肽(CART)作为影响猪繁殖机能的多肽,依据NCBI上登录的CARTmRNA序列设计3对特异性引物,用RT-PCR方法从下丘脑内扩增出CART mRNA的部分序列并进行序列分析；再根据扩增出的序列设计一对带酶切位点的特异性引物,用PCR方法扩增出含EcoR Ⅰ/HindⅢ酶切位...
|
| 关 键 词： | 猪 可卡因苯异丙胺调控转录肽(CART) 载体 表达 |
Cloning of the sequence of CDS region of pig CART mRNA and construction of the expression vector |
| |
| LI Peng-fei,LI Fu-lu,YU Xiu-ju,L Li-hua.Cloning of the sequence of CDS region of pig CART mRNA and construction of the expression vector[J].Journal of Fujian Agricultural and Forestry University,2011,40(6):610-613. |
| |
| Authors: | LI Peng-fei LI Fu-lu YU Xiu-ju L Li-hua |
| |
| Institution: | LI Peng-fei,LI Fu-lu,YU Xiu-ju,L(U) Li-hua |
| |
| Abstract: | Designing three pairs specific primers based on the CART mRNA sequence included in NCBI GenBank,CART mRNA sequences were amplified via RT-PCR from porcine hypothalamus tissue,and were analyzed.According to the amplified sequence,we designed a pair of expressing primers,amplified the whole coding sequence(CDS) domain of CART including EcoRⅠ/HindⅢ digestion sites.The results showed that: the length of CDS in porcine hypothalamus CART mRNA is 367 bp,CART gene can express in the hypothalamus,and it had successfully constructed prokaryotic expression vector of pEG-32a-CART. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
|
