| 平菇、杏鲍菇和白灵菇菌丝多糖对·OH、DPPH·和NO_2~-的体外清除作用 |
| |
| 引用本文： | 刘海英,张运峰,范永山,刘青.平菇、杏鲍菇和白灵菇菌丝多糖对·OH、DPPH·和NO_2~-的体外清除作用[J].中国农学通报,2010,26(17):26-30. |
| |
| 作者姓名： | 刘海英 张运峰 范永山 刘青 |
| |
| 作者单位： | 1. 唐山市农业科学研究院,河北唐山,063000
2. 唐山师范学院生命科学系,河北唐山,063000 |
| |
| 基金项目： | 河北省科技支撑计划项目"蔬菜种质创新和新品种选育" |
| |
| 摘 要： | 为明确平菇、白灵菇和杏鲍菇菌丝多糖对·OH、DPPH·和NO2-自由基的清除能力。利用热水浸提法提取菌丝多糖,Sevag法纯化多糖,利用多功能酶标仪测定对·OH、DPPH·(1,1-diphenyl-2-picrylhydrazyl)、NO2-的清除率,利用DPS软件和MicroOrigin软件进行数据分析。结果表明,在200～2000μg/mL浓度范围内,白灵菇B10、杏鲍菇PL7和平菇P89菌丝多糖对DPPH·的清除率为5.98%～51.96%,EC50值分别为1.98、2.74和3.28mg/mL;对·OH清除率为43.51%～98.09%,PL7的EC50值溢出最小值,B10和P89的EC50值分别为49.6μg/mL和467.3μg/mL;对NO2-的清除率为10.22%～26.75%,P89的EC50值为5.3mg/mL,PL7和B10的菌丝多糖浓度高于500μg/mL时,清除率不再增加,最高值分别为18.53%和17.52%。菌种间抗氧化能力差异明显,同一菌株对不同自由基的清除能力也存在显著差异。3种供试菌种多糖具有较强的清除·OH能力,中等的清除DPPH·能力和较差的清除NO2-能力。杏鲍菇PL7和白灵菇B10菌丝多糖的清除·OH和DPPH·能力较强,而平菇P89具有较强的清除NO2-能力。
|
| 关 键 词： | 小麦 小麦 条锈病 抗性基因 分子标记 |
| 收稿时间： | 2010/3/23 0:00:00 |
| 修稿时间： | 2010/4/29 0:00:00 |
In vitro Clearance of ·OH、DPPH·and NO2-with Polysaccharides from Mycelia of Pleurotus ostreatus,P.nebrodensis and P.eryngii |
| |
| Liu Haiying,Zhang Yunfeng,Fan Yongshan,Liu Qing.In vitro Clearance of ·OH、DPPH·and NO2-with Polysaccharides from Mycelia of Pleurotus ostreatus,P.nebrodensis and P.eryngii[J].Chinese Agricultural Science Bulletin,2010,26(17):26-30. |
| |
| Authors: | Liu Haiying Zhang Yunfeng Fan Yongshan Liu Qing |
| |
| Institution: | 1Tangshan Agricultural Science Academy, Tangshan Hebei 063000; 2Tangshan Teacher’s College, Tangshan Hebei 063000) |
| |
| Abstract: | This paper is to determine the in vitro clearance ability of·OH, DPPH·and NO2-with the mycelial polysaccharides from Pleurotus ostreatus , P. nebrodensis and P. eryngii . Mycelial polysaccharides were extracted by hot water and purified by Sevag method. The anti-oxidation activity was determined by polyfunctional microplate spectrophotometer. The data were analyzed by DPS V7.05 software. The results showed that the clearance rates of ·OH, DPPH· and NO2-were 43.51%-98.09% , 5.98%-51.96% and 10.22%-26.75% respectively in the polysaccharide concentration range of 200-2000 μg/mL. The EC50 values of DPPH·clearance rate for B10, PL7 and P89 were 1.98, 2.74 and 3.28 mg/mL respectively. The EC50 values of·OH clearance rate for P89 and B10 were 49.6 μg/mL and 467.3 μg/mL respectively. The EC50 value of· OH clearance rate for PL7 was beyond the minimum limit. The EC50 value of NO2-clearance rate for P89 was 5.3 mg/mL; The maxima of NO2-clearance rate for PL7 and B10 were 18.53% and 17.52% respectively when the polysaccharide concentration was above 500 μ g/mL. The anti-oxidation activity is different between mushrooms and for different free radicals. The tested mushrooms have high ability to clear·OH, middle ability to clear DPPH·but weak ability to clear NO2-. PL7 and B10 have high clearance ability to·OH and DPPH·, but P89 is at the advantage of NO2-clearance ability. |
| |
| Keywords: | Pleurotus ostreatus P nebrodensis P eryngii mycelial polysaccharides in vitro clearance free radicals |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
