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玉米NF-YC亚基基因的鉴定与表达规律分析
引用本文:王雪,李瑾,朱丽,张悦,藏金萍,张康,邢继红,董金皋.玉米NF-YC亚基基因的鉴定与表达规律分析[J].植物病理学报,2022,52(1):123-128.
作者姓名:王雪  李瑾  朱丽  张悦  藏金萍  张康  邢继红  董金皋
作者单位:河北农业大学 生命科学学院,保定 071000;
河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室,保定 071000
基金项目:国家玉米产业技术体系(CARS-02-12);
摘    要:<正>0 引言NF-Y(nuclear factor-Y, NF-Y)是一类与CCAAT基序结合的转录因子,由NF-YA、NF-YB和NF-YC 3个亚基组成1-2]。NF-YC参与并调控植物生长发育的进程以及在生物和非生物胁迫响应过程中起作用3]。AtNF-YC1通过与AtXTH21基因启动子区的CCAAT-box结合调控拟南芥对冷胁迫的响应4]。AtNF-YC4可响应病原菌的侵染,其过表达植株对病原菌的敏感性降低5]。

收稿时间:2021-01-13

Identification and expression analysis of NF-YC subunit genes in Zea mays
WANG Xue,LI Jin,ZHU Li,ZHANG Yue,ZANG Jinping,ZHANG Kang,XING Jihong,DONG Jingao.Identification and expression analysis of NF-YC subunit genes in Zea mays[J].Acta Phytopathologica Sinica,2022,52(1):123-128.
Authors:WANG Xue  LI Jin  ZHU Li  ZHANG Yue  ZANG Jinping  ZHANG Kang  XING Jihong  DONG Jingao
Institution:College of Life Science, Hebei Agricultural University, Baoding 071000, China;
Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University, Baoding 071000, China
Abstract:NF-Y (nuclear factor-Y) family genes play an important role in plant growth, development and response to stress. Related studies on NF-YC subunit genes of maize have not been reported. In this study, 17 NF-YC subunit genes in silicon cloning and bioinformatics methods were divided into three subfamilies, encoding product of CBFD_NFYB_HMF domain by conserved domain analysis. During maize different developmental stages and different tissues, NF-YC subunit genes exhibited significantly different expression levels. The maize NF-YC subunit genes were detected with obvious differences in their expression level under different abiotic stresses and Fusarium Verticillioide infection. Based on qRT-PCR results, the expression levels of NF-YC subunit genes were significantly changed after SA, JA and ET hormones treatments. Collectively, NF-YC subunit genes display conserved functions in maize growth and development as well as resistance to abiotic and biotic stresses, laying a foundation for elucidating the mechanism of NF-YC subunit genes in maize.
Keywords:Zea mays  NF-YC subunit genes  biotic stress  abiotic stress  expression analysis  
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