Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench) |
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| Authors: | Jianming Gao Buxian Xia Feng Luo Shoujun Sun Zhongyou Pei Zhi Gui Qinghua Yuan Xianglin Li |
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| Institution: | 1. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, People’s Republic of China
2. The Key Laboratory of Crop Genetics and Breeding, Department of Agronomy, Tianjin Agricultural College, Tianjin, 300384, People’s Republic of China
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| Abstract: | A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F5/BC3) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future. |
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