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| 怀牛膝多糖对雏鸡疫苗免疫效果的影响 | |
| 引用本文： | 邱妍,胡元亮,崔保安,张红英,王远阁.怀牛膝多糖对雏鸡疫苗免疫效果的影响[J].畜牧兽医学报,2007,38(7):723-727. |
| 作者姓名： | 邱妍 胡元亮 崔保安 张红英 王远阁 |
| 作者单位： | 1. 南京农业大学动物医学院,南京,210095;河南科技大学动物科技学院,洛阳,471004 2. 南京农业大学动物医学院,南京,210095 3. 河南农业大学牧医工程学院,郑州,450002 |
| 基金项目： | 国家“十五”食品安全重大攻关专项（3001BA804A30-11） |
| 摘 要： | 150羽14日龄蛋公鸡随机均分成3组,用新支二联弱毒苗点眼滴鼻免疫的同时,2个试验组分别肌肉注射高、低剂量的怀牛膝多糖溶液,对照组注射生理盐水,每天注射1次,连续注射3次。分别于免疫后第7、14、21、28、35和42天各组随机抽取8只鸡翼下静脉采血,分离血清,用微量法测定新城疫血凝抑制（HI）抗体效价,于免疫后第10、20、30、40和50天每组随机抽取5只鸡心脏采血,分离淋巴细胞,用MTT法测定淋巴细胞增殖的动态变化,流式细胞仪双染色法检测CD^4＋、CD^8＋T淋巴细胞含量,并放血致死称取体重和胸腺、脾脏、法氏囊的质量,计算器官指数。结果表明,怀牛膝多糖高剂量组的HI抗体效价、CD^4＋/CD^8＋比值和免疫器官指数都明显高于对照组,并且高剂量怀牛膝多糖可以显著促进T淋巴细胞增殖。提示怀牛膝多糖对鸡新支二联疫苗的免疫效果有明显的增强作用。 |
| 关 键 词： | 怀牛膝多糖 新支二联疫苗 抗体 T淋巴细胞 免疫器官 |
| 文章编号： | 0366-6964（2007）07-0723-05 |
| 修稿时间： | 2006-09-11 |
Effects of Achyranthes Bidentata Polysaccharide on Immune Efficacy of Vaccine in Chickens |
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| QIU Yan,HU Yuan-liang,CUI Bao-an,ZHANG Hong-ying,WANG Yuan-ge.Effects of Achyranthes Bidentata Polysaccharide on Immune Efficacy of Vaccine in Chickens[J].Acta Veterinaria et Zootechnica Sinica,2007,38(7):723-727. | |
| Authors: | QIU Yan HU Yuan-liang CUI Bao-an ZHANG Hong-ying WANG Yuan-ge |
| Institution: | 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Animal Science and Technology, Henan Science and Technology University, Luoyang 471004, China;3. College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002,China |
| Abstract: | 150 14-day-old chickens were randomly assigned to three groups and vaccinated with ND-IB live virus vaccine.At the same time,the chickens in two experimental groups were intramuscularly injected with achyranthes bidentata polysaccharide at high and low dosages,control group were injected with physiological saline,once a day for three successive days.On day 7,14,21,28,35 and 42 after vaccination,the dynamic changes of serum Newcastle disease(ND) hemagglutination inhibition(HI) antibody titer were determined by Micro-method.On day 10,20,30,40 and 50 after vaccination,blood samples from 5 chickens per group were collected to separate lymphocyte and to determine the peripheral T lymphocyte proliferation with MTT method.The content of CD4 and CD8 T cells were tested by using flow cytometry with double-color-staining method,and the weights of whole body,spleen,bursa and thymus were weighted for calculation of immune organ index.The results showed that achyranthes bidentata polysaccharide at high dosage significantly enhanced the antibody titers,promoted the proliferation of peripheral T lymphocyte,and raised the ratio of CD4 to CD8 T lymphocyte(P < 0.05) and immune organ indexes.These results indicated that achyranthes bidentata polysaccharide had significant immune enhancement role on immunity of ND-IB vaccine in chickens. |
| Keywords: | achyranthes bidentata polysaccharide ND-IB vaccine antibody T lymphocyte immune organs |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |