| '辽宁2号'核桃品种矮化机制初探 |
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| 引用本文： | 宋晓波,陈凌娜,王红霞,裴东.'辽宁2号'核桃品种矮化机制初探[J].林业科学研究,2015,28(6):910-915. |
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| 作者姓名： | 宋晓波 陈凌娜 王红霞 裴东 |
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| 作者单位： | 中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091;中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091;河北农业大学山区研究所, 河北保定 071001;中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091 |
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| 基金项目： | 国家自然科学基金项目"核桃埋干复幼促根过程中生长素响应基因的CpG岛甲基化重编程研究(31171933) |
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| 摘 要： |
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| 关 键 词： | 核桃 辽宁2号 矮化 SSR位点 JrGAI |
| 收稿时间： | 2015/7/22 0:00:00 |
Dwarfing Mechanism of Dwarfed Walnut Cultivar 'Liaoning 2' |
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| SONG Xiao-bo,CHEN Ling-n,WANG Hong-xia and PEI Dong.Dwarfing Mechanism of Dwarfed Walnut Cultivar 'Liaoning 2'[J].Forest Research,2015,28(6):910-915. |
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| Authors: | SONG Xiao-bo CHEN Ling-n WANG Hong-xia and PEI Dong |
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| Institution: | Research Institute of Forestry, Chinese Academic of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academic of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Mountain Areas Research Institute of Hebei Province, Agricultural University of Hebei, Baoding 071001, Hebei, China;Research Institute of Forestry, Chinese Academic of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
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| Abstract: | 'Liaoning 2' is a dwarf walnut variety bred by Chinese scientists through artificial hybridization, but little is known about its dwarfing mechanism. In this study, the dwarfing mechanism of 'Liaoning 2' is discussed through the comparison of biological characteristics, genomics and the expression of growth-related gene JrGAI,with a growth normally, closely related(half-sib families) variety of 'Liaoning 1' as control. By observing the phenology and tree phenotypes, it was found that there was no significant difference in phenological phase between 'Liaoning 1' and 'Liaoning 2', but 'Liaoning 2' was obviously dwarf, the height, crown and trunk diameter were smaller, the average length of internode reduced significantly, but there was no significant difference in the number of development branch internode. The dwarf phenotype of 'Liaoning 2' is mainly caused by inter-shortening. The qRT-PCR technique was performed to analyze the expression of GA negative regulation gene JrGAI and the results showed that during the shoot development, the expression of JrGAI gene exhibited decreasing expression in the shoot rapid growing period in both varieties and the decline in 'Liaoning 1' was remarkably reduced. The SSR analysis with 25 pairs of primers showed that SSR loci of two varieties were different, especially the homozygosity of 'Liaoning 2' SSR loci was higher. It is concluded that different expressions of JrGAI gene and homozygosity of SSR loci could be important internal reasons of dwarf phenotype of 'Liaoning 2'. |
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| Keywords: | walnut 'Liaoning 2' dwarfing SSR loci JrGAI |
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