| 3个大豆品种及其骨干亲本的亲缘关系和指纹图谱分析 |
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| 引用本文： | 杨加银,徐海风,程保山.3个大豆品种及其骨干亲本的亲缘关系和指纹图谱分析[J].中国农学通报,2015,31(36):65-69. |
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| 作者姓名： | 杨加银 徐海风 程保山 |
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| 作者单位： | (江苏徐淮地区淮阴农业科学研究所/江苏省现代作物生产协同创新中心/国家大豆改良中心淮安试验站,江苏淮安 223001） |
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| 基金项目： | 国家高技术研究发展计划（863计划）项目“强优势大豆杂交种的创制与应用”(2011AA10A105)；江苏省科技支撑计划项目“优质高产高油大豆种质创新与新品种选育”(BE2013350)；淮安市科技计划项目“淮安市盖钧镒院士工作站建设”(HAP201320)。 |
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| 摘 要： | 为了研究江苏淮北地区大豆推广品种及其骨干亲本的亲缘关系和指纹图谱，以3个淮豆系列品种及其8份骨干亲本品种为试验材料，利用CTAB法提取基因组DNA，采用SSR分子标记对11份大豆品种进行亲缘关系和指纹图谱分析。结果显示：利用筛选出的46对SSR引物在11个大豆品种中共检测出125个等位变异，每对引物可检测到2~5个等位变异。使用非加权类平均法进行聚类分析，大部分供试材料间的遗传变异较小，遗传相似系数为0.5781~0.9609。SSR标记遗传距离为0.0391~0.4219，平均0.2773。从上述引物中选取5个核心引物构建的指纹图谱能够鉴别3个淮豆系列品种。上述结果不仅用于品种鉴定及其知识产权保护，还为有效选配亲本拓宽遗传基础提供了理论基础和技术支持。
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| 关 键 词： | 辽河流域 辽河流域 韦伯-费希纳定律 水环境 预警 |
| 收稿时间： | 2015/9/14 0:00:00 |
| 修稿时间： | 2015/10/10 0:00:00 |
Analysis of Genetic Relationship and DNA Fingerprinting of Three Soybean Cultivars and Their Core Parental Lines |
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| Yang Jiayin,Xu Haifeng and Cheng Baoshan.Analysis of Genetic Relationship and DNA Fingerprinting of Three Soybean Cultivars and Their Core Parental Lines[J].Chinese Agricultural Science Bulletin,2015,31(36):65-69. |
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| Authors: | Yang Jiayin Xu Haifeng and Cheng Baoshan |
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| Institution: | (Huaiyin Institute of Agricultural Sciences of Xuhuai Region/Jiangsu Collaborative Innovation Center for Modern Crop Production/Huaian Experimental Station of National Center for Soybean Improvement, Huaian Jiangsu 223001) |
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| Abstract: | In order to study genetic relationship and DNA fingerprinting of commercial soybean cultivars and their patental lines in Huaibei Region, Jiangsu, total genomic DNA of 3 high-yielding (Huaidou) soybean cultivars and their 8 core parental lines was extracted by using CTAB method. The genetic similarity coefficients and DNA fingerprinting of the above-mentioned materials were conducted by using SSR markers. 46 pairs of primers were selected and distributed evenly on the whole genome, of which 2 to 5 alleles were detected by each pair of primers. A total of 125 polymorphism fragments were amplified, whose average number was 2.7 per pair of primers. The cluster analysis was conducted by using unweighted pair-group method with arithmetic means (UPGMA). The genetic variation in most of the experimental materials was lower, and their genetic similarity coefficients changed from 0.5781 to 0.9609. The genetic distance between SSR markers were from 0.0391 to 0.4219 and their average value was 0.2773. The fingerprinting of 5 core primers selected from the above primers could be markedly distinguished between the 3 high-yielding (Huaidou) cultivars. These results not only can be used to detect the cultivars (lines) authenticity and protect intellectual property rights, but also to provide theoretical basis and technical support for effective selection of parents to broaden the genetic basis. |
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| Keywords: | soybean high-yielding cultivars core parental line fingerprinting SSR |
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