| 纤维素降解细菌的筛选及其酶活测定 |
| |
| 引用本文： | 蒋明星,丁晓帆.纤维素降解细菌的筛选及其酶活测定[J].中国农学通报,2015,31(36):161-164. |
| |
| 作者姓名： | 蒋明星 丁晓帆 |
| |
| 作者单位： | (;1.海南大学环境与植物保护学院,海口 570228；;2.云南大学微生物研究所,昆明 650091） |
| |
| 基金项目： | 中西部高校项目“热带有害生物生物学及绿色防控关键技术与集成”(ZXBJH-XK005)。 |
| |
| 摘 要： | 为了充分利用植物废渣中丰富的纤维素资源，以羧甲基纤维素钠(CMC-Na)为唯一碳源的培养基和刚果红染色筛选法从朽木周围富含腐殖质的土壤中筛选得到3株活性较高的纤维素降解细菌：ZWY-3、DP-6和DP-8，并分别在不同的培养时间、培养温度、起始pH、碳源下对3株菌株进行酶活测定。结果表明：3株菌株都在接种后24 h、pH 7、30℃、葡萄糖为碳源时达到产酶高峰，其中接种后24 h时DP-6的酶活最高，达144.86 U；ZWY-3在30℃、pH 7时酶活最大，分别达126.74 U和133.82 U；3株菌株在其最适条件下培养，并以葡萄糖为碳源时，DP-6活性最高，可达218.96 U。3株细菌菌株在降解农业纤维工业中具有良好的应用前景。
|
| 关 键 词： | 农村金融 农村金融 资源配置 效率 黑龙江省 |
| 收稿时间： | 9/9/2015 12:00:00 AM |
| 修稿时间： | 2015/11/24 0:00:00 |
Screening of Cellulose-decomposing Bacteria and Enzymatic Activity Determination |
| |
| Jiang Mingxing and Ding Xiaofan.Screening of Cellulose-decomposing Bacteria and Enzymatic Activity Determination[J].Chinese Agricultural Science Bulletin,2015,31(36):161-164. |
| |
| Authors: | Jiang Mingxing and Ding Xiaofan |
| |
| Institution: | (;1.College of Environment & Plant Protection, Hainan University, Haikou 570228;2.Yunnan Institute of Microbiology, Yunnan University, Kunming 650091) |
| |
| Abstract: | In order to take full advantage of cellulose in plant residues, three strains (ZWY-3, DP-6 and DP-8) of cellulose-decomposing bacteria were isolated from the humus-rich soil by the medium with the sole carbon source of CMC-Na and Congo red screening method. Enzyme activity was measured under different incubation time, temperature, original pH and carbon source. The results showed that three strains showed the highest cellulose activity under the condition of 24 h, pH 7, 30℃ and glucose as carbon source, DP-6 had the highest cellulose activity of 144.86 U when the incubation time was 24 h, ZWY-3 had the cellulose activity of 126.74 U when the incubation temperature was 30℃, and the highest cellulose activity of 133.82 U when the pH value was 7. DP-6 showed the highest activity of 218.96 U when cultivated under the optimal condition with glucose as the only carbon source. These three strains showed great potential in the industry of agricultural cellulose-decomposing. |
| |
| Keywords: | cellulose-decomposing bacteria isolation cellulose activity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
