Leaf structure and chemical compositions are correlated with cotton boron efficiency |
| |
| Authors: | Wu Xiuwen Dong Xiaochang Lu Xiaopei Muhammad Riaz Jiang Cuncang |
| |
| Institution: | 1. Microelement Research Center, Huazhong Agricultural University, Wuhan, China;2. Key Laboratory of Arable Land Conservation, Ministry of Agriculture, Wuhan, China |
| |
| Abstract: | In this study, in order to investigate the boron (B) efficiency and response to changes in leaves structure and chemical compositions correlated with B efficiency, two different cotton genotypes including genotype 103 and genotype 122 were treated by B-deficiency (0 mg L?1) or moderate B (0.2 mg L?1). All the seedlings were grown in hydroponics situation with modified Hoagland and Arnon solution under greenhouse conditions for 6 weeks. The changes in chemical compositions and cell structure of leaves as a consequence of B deficiency were revealed by Fourier-transform infrared spectroscopy (FTIR), 13C nuclear magnetic resonance (13C-NMR) and transmission electron microscope (TEM). The results showed that the leaves of genotype 122 appeared crinkled and prolapsed having more obvious symptoms than genotype 103. Besides, the dry mass of every part was higher while B concentration was lower in genotype 103, indicating that genotype 103 was much tolerant to low B, which was related to higher B utilization efficiency of genotype 103 under B-deprived condition. In addition, B deficiency stunted the synthesis of proteins, carbohydrates, cellulose and lignin, which is noticed more seriously in leaves of genotype 122, and the structure and integrity of cells were significantly destroyed by B starvation. These results indicated that genotype 103 had higher B efficiency than genotype 122, and the changes in the chemical composition and cell structure might be either specific or adaptive responses to B efficiency. |
| |
| Keywords: | cell structure 13C-NMR efficiency FTIR leaf composition physiology |
|
|
