| 荧光分子信标探针检测双歧杆菌的研究 |
| |
| 引用本文： | 王超,孟祥晨.荧光分子信标探针检测双歧杆菌的研究[J].东北农业大学学报,2008,39(3):100-104. |
| |
| 作者姓名： | 王超 孟祥晨 |
| |
| 作者单位： | 乳品科学教育部重点实验室,东北农业大学食品学院,哈尔滨,150030 |
| |
| 摘 要： | 根据双歧杆菌高度保守的寡核苷酸序列,构建一枚荧光分子信标探针,并对探针的性能进行测定分析,利用实时PCR技术对双歧杆菌进行检测。研究结果表明,所构建的分子信标设计合理,合成正确,性能稳定,S/N>20,其发卡既可以闭合也可以打开,符合实时PCR过程对分子信标的要求;该信标探针对双歧杆菌具有很好的稳定性及特异性,通过实时PCR扩增能有效地将双歧杆菌和非双歧杆菌区别开。因此,荧光分子信标探针可用于对双歧杆菌的检测。
|
| 关 键 词： | 双歧杆菌 分子信标 探针 实时PCR |
| 文章编号： | 1005-9369(2008)03-0100-05 |
| 修稿时间： | 2007年3月13日 |
Detection for Bifidobacterium by molecular beacon |
| |
| WANG Chao,MENG Xiangchen.Detection for Bifidobacterium by molecular beacon[J].Journal of Northeast Agricultural University,2008,39(3):100-104. |
| |
| Authors: | WANG Chao MENG Xiangchen |
| |
| Abstract: | According to conservative 16S rDNA sequence of Bifidobacterium,one fluorescence molecule be-acon probe was designed and characterized,and then used real-time PCR technology to detect bifidobacteria.The results indicated that the molecular beacon probe was stable and rational,whose S/N value was more than 20,and whose hairpin can be both closed and opened up.This fluorescence molecule beacon probe could be used in real time PCR detection;the probe had the very good stability and specificity for Bifidobacterium.The fluorescence molecule beacon probe may be used to detect bifidobacteria. |
| |
| Keywords: | Bifidobacterium molecular beacon probe real-time PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
