| 沙棘籽中原花青素的分离纯化及抗氧化性的研究 |
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| 引用本文： | 鲁长征,任蓓蕾,山永凯.沙棘籽中原花青素的分离纯化及抗氧化性的研究[J].国际沙棘研究与开发,2010,8(3):11-16. |
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| 作者姓名： | 鲁长征 任蓓蕾 山永凯 |
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| 作者单位： | 青海清华博众生物技术有限公司,青海西宁,810016 |
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| 摘 要： | 实验以青海沙棘籽为原料，进行了原花青素的分离纯化及抗氧化性研究。通过正交试验对提取工艺进行优化，得出最佳工艺参数为：料液比1：25、粉碎粒度60目、提取温度80℃、提取时间60min。采用NKA大孔树脂对提取物进行纯化，产品浓度由29．5％提高至95．2％，原花青素回收率为73．9％。对纯化原花青素进行DPPH·、羟自由基、超氧阴离子自由基的清除能力研究，结果表明纯化原花青素具有很强的抗氧化能力。
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| 关 键 词： | 沙棘籽 分离 纯化 抗氧化性 最佳工艺 |
Separation, Purification and Antioxidation of Proanthocyanidins from Seabuckthorn Seeds |
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| Authors: | Lu Changzheng Ren Beilei Shan Yongkai |
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| Institution: | (Qinghai Tsinghua Biotry Bio--Tech Co. , Ltd. , Xining, Qinghai 810016) |
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| Abstract: | Qinghai seabuckthorn seeds was used as raw materials, and researched separation, purifica- tion and antioxidation. By orthogonal test to optimize the extraction process and the best parameters as follows： liquid ratio 1 ： 25, crush size 60 head, extraction temperature 80℃, extraction time 60 min. NKA macroporous resin was used to purify the extract, product concentration improved from 29.5 % to 95.2 %, recovery rate 73.9 %. In addition, the purified proanthocyanidins from seabuck- thorn seed were found to be effective on DPPH ~ radical scavenging, superoxide anion radical and hydrogen radical scavenging. |
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| Keywords: | seahuckthorn seeds separation purification antioxidation optimum technology |
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