Carbohydrase activities of the rolC-gene transformed and non-transformed ginseng cultures |
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Authors: | Bulgakov V P Kusaykin M Tchernoded G K Zvyagintseva T N Zhuravlev Yu N |
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Institution: | Far East Branch of Russian Academy of Sciences, Laboratory of Biotechnology, Institute of Biology and Soil Science, Prop Stoletija, 159, Vladivostok 690022, Russia. biotech@eastnet.febras.ru |
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Abstract: | The levels of activity of beta-D-glucosidase, alpha-D-mannosidase, alpha- and beta-D-galactosidase, 1,3-, 1,6-, 1,4-beta-D-glucanases, 1,4-alpha-D-glucanase, fucoidanhydrolase and agarase were measured in extracts of non-transgenic and transgenic Panax ginseng cultures transformed with the rolC gene. Significantly increased levels of activity of beta- and alpha-D-galactosidases and 1,3-beta-D-glucanase were detected in rolC-gene transformed cells, compared to the control non-transformed cells, while levels of activity of other enzymes were unchanged. These, as well as the gel-permeation experiments, revealed that transformation of ginseng cells by the rolC gene could significantly affect activity of some carbohydrases and production of their molecular forms. |
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