| 马铃薯脱毒试管苗污染控制效果研究 |
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| 引用本文： | 康萍芝,张丽荣,詹虹,沈瑞清,郭成瑾.马铃薯脱毒试管苗污染控制效果研究[J].北方园艺,2011(20):134-136. |
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| 作者姓名： | 康萍芝 张丽荣 詹虹 沈瑞清 郭成瑾 |
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| 作者单位： | 宁夏农林科学院植物保护研究所,宁夏植物病虫害防治重点实验室,宁夏银川750002;宁夏马铃薯脱毒种薯繁育中心,宁夏平吉堡,750024 |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2009BADC5B04) |
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| 摘 要： | 通过在MS培养基中加入抗生素和杀菌剂进行逐步筛选,解决马铃薯脱毒试管苗快繁过程中的污染问题.结果表明:防止马铃薯脱毒试管苗污染的最佳抑茵剂组合MS+ 50 mg/L硫酸庆大霉素+50 mg/L氨苄青霉素+50 mg/L硫酸链霉素+0.4g/L多茵灵,该组合有效降低了马铃薯脱毒苗细菌和真菌污染,提高了快繁效率.
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| 关 键 词： | 马铃薯脱毒苗 污染 抑菌剂 控制效果 |
Study on the Contamination Control Effect of Virus-free Potato Plantlets |
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| KANG Ping-zhi
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ZHANG Li-rong
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ZHAN Hong
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SHEN Rui-qing
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GUO Cheng-jin.Study on the Contamination Control Effect of Virus-free Potato Plantlets[J].Northern Horticulture,2011(20):134-136. |
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| Authors: | KANG Ping-zhi ZHANG Li-rong ZHAN Hong SHEN Rui-qing GUO Cheng-jin |
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| Institution: | KANG Ping-zhi1,ZHANG Li-rong1,ZHAN Hong2,SHEN Rui-qing1,GUO Cheng-jin1(1.Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Ningxia Key Laboratory for Control of Plant Disease and Insect Pest,Yinchuan,Ningxia 750002,2.Ningxia Propagation Center of Virus-free Seed Potatoes,Pingjipu,Ningxia 750024) |
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| Abstract: | In order to solve contamination problem of virus-free potato plantlets in rapid propagation,this experiment by joining antibiotics and fungicide in MS medium gradually screened and choosed.The results showed that best antibacterial agent preventing the virus-free potato plantlets contamination,namely,MS+50 mg/L Gentamycin Sulfate+50 mg/L Ampicillin Sodium+50 mg/L Streptomycin Sulfate+0.6 g/L carbendazim.Through this way,bacteria and fungi contamination of virus-free potato plantlets were reduced effectively... |
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| Keywords: | virus-free potato plantlets contamination antibacterial agent control effect |
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