| 微胶囊剂型黄芪多糖对刺参生长性能、免疫力及抗病力的影响 |
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| 作者姓名： | 樊英 王淑娴 李天保 许拉 于晓清 叶海斌 刁菁 王勇强 |
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| 作者单位： | 山东省海水养殖研究所病害防治重点实验室 |
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| 基金项目： | 山东省农业重大应用技术创新课题(鲁财农指[2010]58号);海洋公益性行业科研专项经费项目200905020;山东省科技发展计划项目(2010GHY10501);山东省优秀中青年科学家科研奖励基金项目(BS2010NY021)共同资助 |
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| 摘 要： | 在基础饲料中添加不同剂量(1%、3%、5%)的微胶囊剂型黄芪多糖(Astragalus polysac-charudes,APS)(载药量为17%),制成3种配合饲料,连续投喂初始体重为22.0±1.0g的刺参Apostichopus japonicus35d,探讨微胶囊剂型黄芪多糖(以下简称"微胶囊")对刺参生长性能、非特异性免疫及抗病力的影响。结果显示,35d后刺参最高特定生长率(Specific growth rate,SGR)为0.51%/d(5%添加组)(P<0.05);微胶囊添加量为3%时免疫效果最佳,刺参体腔液中酸性磷酸酶ACP、超氧化物歧化酶SOD、髓过氧化物酶MPO活性显著高于对照组(P<0.05)。试验期间每隔7d测定刺参体腔液样品,其中ACP活性在第28天最高,为11.2U/100ml(P<0.05);SOD活性在第21天最高,为63.3U/ml(P<0.05);MPO活性在第21天最高,为40MPO单位/ml(P>0.05);免疫试验结束后对刺参进行灿烂弧菌Vibrio splendidus攻毒实验,14d内观察得出,3%微胶囊添加组累积死亡率明显低于对照组(P<0.05)。由此可得,微胶囊剂型黄芪多糖对仿刺参生长性能、免疫力及抗病力方面均具有显著的增强效果,以3%添加量效果最佳,且添加量、作用时间、免疫效果之间存在相关性。
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| 关 键 词： | 微胶囊 黄芪多糖 刺参 生长 免疫 抗病力 |
| 收稿时间： | 2012/5/18 0:00:00 |
| 修稿时间： | 2012/5/30 0:00:00 |
Impact of APS microcapsules on growth,immunity and disease resistance of sea cucumber Apostichopus japonicus |
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| Authors: | FAN Ying WANG Shu-xian LI Tian-bao XU L YU Xiao-qing YE Hai-bin DIAO Jing WANG Yong-qiang |
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| Institution: | (Key Laboratory for Disease Control in Mariculture,Shandong Province Mariculture Research Institute,Qingdao 266002) |
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| Abstract: | The study was conducted to investigate the impact of Astragalus polysaccgarudes(APS) microcapsules (drug loading 17%) on the growth, nonspecific immune indices and resistance of Apostichopus japonicas against Vibrio splendidus infection.APS microcapsules were added at graded levels (1%, 3%, and 5%) to the basal diet to formulate three practical compound diets. Continuous 35-day feeding trials were carried out on A. japonicus at initial body weight of 22.0±1.0g. After feeding the diets for 35 days, specific growth rate (SGR) for the 5% treatment was 0.51%/d, which was significantly higher than the control. The activities of serum acid phosphatase (ACP), superoxide dismutase (SOD), and myeloperoxidase (MPO) increased significantly (P<0.05) in the 3% treatment. During the experiment these factors were determined at 7-day intervals. The activity of ACP was 11.2U/100ml at 28d during the experiment (P<0.05), the activity of SOD was 63.3U/ml at 21d (P<0.05), and the activity of MPO was 40MPO unit/ml at 21d (P>0.05). After the feeding trial, a V. splendidus injection challenge was given to the sea cucumbers to test the effects of APS microcapsule. The 3% treatment showed significantly better survival than the control (P<0.05). It is suggested that supplementation of 3% APS microcapsules has the greatest effects on growth, immunity and disease resistance in A. japonicas against V.splendidus. In addition, there was a direct relationship between the level and time of treatment, and the immunity. |
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