| 鸭坦布苏病毒RT-PCR诊断方法的建立与应用 |
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| 引用本文： | 李国平.鸭坦布苏病毒RT-PCR诊断方法的建立与应用[J].中国畜牧兽医,2013,40(1):69-72. |
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| 作者姓名： | 李国平 |
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| 作者单位： | 青海省大通县斜沟乡畜牧兽医站, 青海大通 810100 |
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| 摘 要： | 为建立一种快速的鸭坦布苏病毒病原检测方法,本研究根据GenBank上登录的鸭坦布苏病毒(duck Tembusu virus,DTMUV)基因组序列,设计1对引物,建立了检测DTMUV的RT-PCR诊断方法。该方法对Ⅰ型鸭肝炎病毒、新城疫病毒、减蛋综合征病毒、鸭肠炎病毒等常见鸭病的扩增结果均为阴性,检测的敏感性达1 ng RNA。该RT-PCR方法具有良好的特异性、敏感性、重复性,可以准确快速检测出极低含量的DTMUV,将为DTMUV的病原检测及分子流行病学调查等提供一种快速、灵敏、特异、准确的分子生物学检测方法。
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| 关 键 词： | 鸭坦布苏病毒 RT-PCR 诊断 |
| 收稿时间： | 2012-06-28 |
Development and Application of RT-PCR Method for Detection of Duck Tembusu Virus |
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| LI Guo-ping.Development and Application of RT-PCR Method for Detection of Duck Tembusu Virus[J].China Animal Husbandry & Veterinary Medicine,2013,40(1):69-72. |
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| Authors: | LI Guo-ping |
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| Institution: | Husbandry and Veterinary Station of Xiegou Town in Datong County, Datong 810100, China |
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| Abstract: | To establish a rapid duck Tembusu virus(DTMUV)pathogen detection methods, based on DTMUV gene sequence in GenBank, we synthesized one pair of primers, established a RT-PCR for DTMUV detection. The method was used for detecting duck hepatitis virus (DHV-Ⅰ), Newcastle disease virus(NDV), egg drop syndrome virus(EDSV), duck enteritis virus (DEV), all of the PCR results were negative, the sensitivity was 1 ng RNA, the established RT-PCR method had good specificity, sensitivity, reproducibility, it could detect very low levels of DTMUV quickly and accurately, it provided a kind of rapid, sensitive, specific and precise molecular biology detection method for pathogen detection and molecular epidemiology of material such as DTMUV. |
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| Keywords: | duck Ttembusu virus RT-PCR diagnosis |
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