| 培养基种类、玉米素浓度及pH值对蓝莓“美登”组培增殖生长的影响 |
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| 引用本文： | 廉家盛,朴炫春,廉美兰,田娇.培养基种类、玉米素浓度及pH值对蓝莓“美登”组培增殖生长的影响[J].延边大学农学学报,2010,32(4):269-272. |
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| 作者姓名： | 廉家盛 朴炫春 廉美兰 田娇 |
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| 作者单位： | 延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 |
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| 基金项目： | 延边大学研究生创新科研项目 |
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| 摘 要： | 以蓝莓"美登"品种茎段为外植体,研究基本培养基种类、ZT浓度和培养基pH值对其组培苗增殖的影响,结果表明,B5培养基比MS、White和改良WPM更适于"美登"不定芽增殖培养,当B5培养基中加入2.0mg/L ZT时,每株可分化出6.8个不定芽,ZT浓度低于或高于2.0 mg/L时,分化芽数降低,"美登"组培苗生长受到抑制.pH值对美登组培苗增殖生长也有影响,pH值为5.4时分化的芽数最多(11.5个/株),且生长良好.
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| 关 键 词： | 蓝莓 不定芽 组织培养 |
Effect of medium types, ziatin concentration and pH on shoot proliferation and growth of Blueberry in vitro |
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| LIAN Jia-sheng,PIAO Xuan-chun,LIAN Mei-lan,TIAN Jiao.Effect of medium types, ziatin concentration and pH on shoot proliferation and growth of Blueberry in vitro[J].Journal of Agricultural Science Yanbian University,2010,32(4):269-272. |
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| Authors: | LIAN Jia-sheng PIAO Xuan-chun LIAN Mei-lan TIAN Jiao |
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| Institution: | (Key Laboratory of Nature Resource and Functional Molecular of Changbai Mountain of Yanbian University,Yanji Jilin 133002,China) |
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| Abstract: | The stems of Blueberry ＂Blomidon＂were used as explant to investigate the kinds of culture medium,the concentration of ZT and pH of medium that affecting the rapid propagation of shoots of Blueberry.The results showed that B5 was more suitable for blueberry in vitro shoot proliferation than MS,White and improved WPM.The B5 supply with 2.0 mg/L ZT differentiated 6.8 shoots and bud differentiation decreased and shoots growth was inhibited when the concentration of ZT below or above 2.0 mg/L.The pH value of medium also had effect on shoots growth and the shoots grew well and the differentiation was up to 11.5/ plant in pH of 5.4. |
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| Keywords: | blueberry proliferation tissue culture |
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