| 甘蔗茎尖细胞有丝分裂过程中微管骨架的变化 |
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| 引用本文： | 李志刚,赵洪波,李素丽,杨丽涛,李杨瑞.甘蔗茎尖细胞有丝分裂过程中微管骨架的变化[J].农业科学与技术,2008,9(1):94-98. |
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| 作者姓名： | 李志刚 赵洪波 李素丽 杨丽涛 李杨瑞 |
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| 作者单位： | [1]广西大学农学院,广两南宁530005 [2]广西农业科学院,广西南宁530007 |
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| 摘 要： | 笔者拟从微管列阵变化及其参与染色体运动的关系方面探讨甘蔗茎增粗机理，为单子叶植物的微管与染色体相关研究提供一定的例证。
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| 关 键 词： | 甘蔗 茎尖细胞 有丝分裂 微管骨架 |
The Change of Microtubule Cytoskeleton in the Stem-Tip Cells of Sugarcane during Mitosis |
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| LI Zhi-gang,ZHAO Hong-bo,LI Su-li,YANG Li-tao,LI Yang-rui.The Change of Microtubule Cytoskeleton in the Stem-Tip Cells of Sugarcane during Mitosis[J].Agricultural Science ＆ Technology,2008,9(1):94-98. |
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| Authors: | LI Zhi-gang ZHAO Hong-bo LI Su-li YANG Li-tao LI Yang-rui |
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| Institution: | LI Zhi-gang, ZHAO Hong-bo, LI Su-li, YANG Li-tao, LI Yang-rui (1.College of Agriculture, Guangxi University, Nanning 530005;2.Guangxi Academy of Agricultural Sciences, Nanning 530007) |
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| Abstract: | In order to understand the microtubule change of monoeotyls stem-tip during mitosis,the arrangement,transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome,indirect immunofluorescence,DAPI staining and fluorescence microscopy.The results showed that nueleolus was intact when the cortical miemtubules formed;cortical mierotubulos were changed into phramoplast microtubule bands at mitosis prophase.When phramoplast microtubule came into being,nuclear membrane was ruptured and chromosome was arranged at the position of cell plate;subsequently,phramoplast microtubules were changed into phragmoplast mierotubules,phramoplast mierotubules were shortening and microtubules on the sides of cell plate were increasing gradually,during this course sister chromatid was separated by microtubules at cell plate and tract to the two poles,forming phragmoplast microtubules.Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers.Therefore,cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status. |
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| Keywords: | Sugarcane Microtubule eytoskeleton Microtubuh cycles Mitosis |
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