| 芦苇草DNA导入小麦抗盐性转化体的筛选 |
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| 引用本文： | 刘志生,张坤普.芦苇草DNA导入小麦抗盐性转化体的筛选[J].吉林农业科学,1998(4):29-31. |
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| 作者姓名： | 刘志生 张坤普 |
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| 摘 要： | 以鲁麦８号小麦为受体，开花授粉０５ｈ后导入芦苇草ＤＮＡ１０穗，１１３朵花。Ｄ０收获６６粒，Ｄ１实收３９个单株。从Ｄ２中选出１６个变异株，出现比较明显变异性状的是无芒、粒色、株高、穗长、穗粒数、颖尖的锐、钝等，特别是变异后代的抗盐性。以上变异后代通过自然盐渍土、盐池、水培筛选，选育出了８９Ｄ２５２２１３、８９Ｄ３１１１１９等抗盐性新品系。从形态特征和性状上看，说明芦苇草ＤＮＡ已进入到鲁麦８号受体细胞核内，并对受体基因组产生了影响，其后代得到充分表达。
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| 关 键 词： | 芦苇草 DNA导入 小麦 抗盐性 筛选 |
Reed DNA Introducing into Wheat and Salt_resistant Transformation Screening |
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| Abstract: | The introduction was made between wheet cultivar Lumai 8 as receptor and reed.After half hour of pollination 113 flowers of 10 ears were used for the introduction.The plants of D 0 genenation produced 66 grains.Then 39 plants were obtained from D 1 genenation and 16 variant plants were selected from D 2 genenation.The evident variations were found in several phenotypes,including the awn property,grain coclor,plant height,ear leath,grain number of each ear and sharp or dull of grain husk point,especially salt resistance.The variation were screened from nature salt soil,salt pond ,water pond planting in late generations 89D 252213 and 89D 311119 and other salt_resistant lines were selected. |
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| Keywords: | Reed chromosome DNA introducing Wheat Variation Screen Salt resistant |
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