| 一个水稻散生突变体tag1的遗传分析及基因定位 |
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| 引用本文： | 杨菲,张德春.一个水稻散生突变体tag1的遗传分析及基因定位[J].农业现代化研究,2015,36(4):684-689. |
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| 作者姓名： | 杨菲 张德春 |
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| 作者单位： | 中国科学院亚热带农业生态研究所 中国科学院亚热带农业生态过程重点实验室，湖南 长沙410125;中国科学院大学，北京100049;三峡大学生物技术研究中心，湖北 宜昌443002,三峡大学生物技术研究中心，湖北 宜昌443002 |
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| 基金项目： | 国家自然科学基金 (31371596) 。 |
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| 摘 要： | 株型对水稻产量有着重要影响。作为影响株型的重要因素之一,分蘖角度是水稻高产育种上考虑的重要农艺性状。散生突变体tag1(tiller angle 1)在水稻品种台北309转基因后代中被发现。与野生型相比,主要突变表型为分蘖角度和叶夹角显著增大,并伴随株高、穗长、结实率和枝梗数等性状上的变化。遗传分析表明,tag1的突变性状由一对隐性基因控制。利用F2(tag1/明恢63)群体作为定位群体,突变基因被定位在第11染色体长臂In Del标记AC35795和M7之间,遗传距离分别为0.38 c M和0.95 c M。在该区间内存在一个已被克隆的控制水稻分蘖角度的基因LAZY1。测序比对分析发现,突变体的TAG1存在一个119 bp的缺失,包括第4个外显子50 bp,第4个内含子69 bp,及二者间的剪切位点。因此TAG1是LAZY1的一个新的等位基因,重新命名为LAZY1-2。lazy1和tag1在突变表型上存在一定差异,这可能是LAZY1基因序列的突变位点不同造成的。
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| 关 键 词： | 水稻 分蘖角度 分子标记 基因定位 LAZY1基因 |
| 收稿时间： | 2014/10/21 0:00:00 |
| 修稿时间： | 2015/4/28 0:00:00 |
Genetic analysis and fine-mapping of a tiller spreading mutant in rice |
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| YANG Fei and ZHANG De-chun.Genetic analysis and fine-mapping of a tiller spreading mutant in rice[J].Research of Agricultural Modernization,2015,36(4):684-689. |
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| Authors: | YANG Fei and ZHANG De-chun |
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| Institution: | Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China;University of Chinese Academy of Sciences, Beijing 100049, China;Biotechnology Research Center, Three Gorges University, Yichang, Hubei 443002, China and Biotechnology Research Center, Three Gorges University, Yichang, Hubei 443002, China |
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| Abstract: | Plant architecture plays an important role in rice(Oryza sativa L.) yield. As a key component of rice plant architecture, tiller angle has been considered as an important agronomic trait in ideal plant architecture and high-yield breeding. The mutant in this study originated from offspring of transgenic plants in japonica rice cultivar TP309. Compared to the wild type, the mutant exhibits wider tiller angle and leaf angle, accompanied with other phenotypic changes such as plant height, panicle length, setting rate and so on. Genetic analysis showed that the tiller spreading trait of the mutant is controlled by one single recessive gene. The F2 mapping population was created by crossing tag1 with Minghui63. The mutant gene was mapped between InDel markers AC35795 and M7, on the long arm of chromosome 11, with genetic distances of 0.38 and 0.95 cM respectively. Within this region, there is a cloned gene LAZY1, which controls rice tillering angle. Sequencing analysis reveals a 50 bp deletion at the fourth exon and a 69 bp deletion at the fifth intron, which leads to the loss of the splice site and the remaining segment direct connection between the fourth intron and the fourth exon in mRNA processing. Therefore, TAG1 is an allele of LAZY1 and is renamed as LAZY1-2. The different phenotypes of the two mutants may be caused by different mutational sites of genomic sequence. |
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| Keywords: | rice tiller angle molecular marker gene mapping LAZY1gene |
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