| 云南半细毛羊毛囊干细胞表面标记物的鉴定 |
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| 引用本文： | 李东江,权国波,杨红远,吕春荣,洪琼花.云南半细毛羊毛囊干细胞表面标记物的鉴定[J].中国草食动物,2014(1):5-7. |
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| 作者姓名： | 李东江 权国波 杨红远 吕春荣 洪琼花 |
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| 作者单位： | 云南省畜牧兽医科学院,昆明650224 |
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| 基金项目： | 国家现代农业产业技术体系建设（CARS-40）;云南省应用基础研究青年项目（2012FD083） |
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| 摘 要： | 设计8对引物（CK14、CK15、CK19、CD34、CD271、CD200、nestin和β1）,分别扩增云南半细毛羊成纤维细胞和毛囊干细胞表面标记物.结果表明：在成纤维细胞中CK19和β1表现为阳性,而在毛囊干细胞中CK14、CK19和β1表现为阳性,其他标记物的表达都为阴性.因此,CK14可以作为鉴定云南半细毛羊毛囊干细胞的表面标记物之一.
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| 关 键 词： | 云南半细毛羊 毛囊干细胞 表面标记物 |
Identification of Hair Follicle Stem Cell Surface Markers From Yunnan Semi-wool Sheep |
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| Li Dong-jiang,Quan Guo-bo,Hong Qiong-hua.Identification of Hair Follicle Stem Cell Surface Markers From Yunnan Semi-wool Sheep[J].China Herbivores,2014(1):5-7. |
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| Authors: | Li Dong-jiang Quan Guo-bo Hong Qiong-hua |
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| Institution: | , et al (Yunnan Academy of Animal and Veterinary Sciences, Kunming 650224, China) |
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| Abstract: | Eight paris of primers （CK14, CK15, CK19, CD34, CD271, CD2OO,nestin and β1 ）were designed to amplify surface markers of hair follicle stem cells and fihrohlast cells from Yunnan semi-wool sheep,the results showed that CK19 and β1 per- formed positive in fibroblast cells, CK14, CKI9 and β1 performed positive in hair follicle stem cells, the expression of other mark- ers were negative, so CK14 could be used as one of the surface markers to identify follicle stem cells of Yunnan semi-wool sheep. |
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| Keywords: | Yunnan semi-wool sheep hair follicle stem cell surface marker |
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