| SYBR Green实时荧光定量PCR检测利玛原甲藻的研究 |
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| 引用本文： | 殷安齐,李洪武,刘志媛,伴修平,田边祥子,细井公富.SYBR Green实时荧光定量PCR检测利玛原甲藻的研究[J].热带作物学报,2010,31(12):2147-2152. |
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| 作者姓名： | 殷安齐 李洪武 刘志媛 伴修平 田边祥子 细井公富 |
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| 作者单位： | 海南大学;海南大学;海南大学;滋贺县立大学;神户大学;福井县立大学 |
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| 基金项目： | 第一作者简介：殷安齐，女，1985年生，硕士研究生。研究方向：热带水生生物病害防治。E-mail：ancky_y_0116@yeah.net。 |
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| 摘 要： | 建立应用SYBR Green实时荧光定量PCR技术快速定量检测利玛原甲藻的方法。结果表明,理论上,该方法对于4个利玛原甲藻细胞便可检测出,灵敏度较高;实际应用时检测出的细胞数为(505±14.72)cells/L(镜检为600 cells/L,误差为15.83%,符合海洋调查规范中浮游生物调查误差在20%以内的要求)。本检测方法具有快速、特异、准确等优点,可作为海洋环境监测中定性定量检测利玛原甲藻的方法。
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| 关 键 词： | 利玛原甲藻 SYBR Green 荧光定量PCR 检测 |
SYBR Green Real-time Fluorescence Quantitative PCR Detection of Prorocentrum lima |
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| Yin anqi,Li hongwu,Liu zhiyuan,Ban syuuhei,Tanabe shouko and Hosoi kungfu.SYBR Green Real-time Fluorescence Quantitative PCR Detection of Prorocentrum lima[J].Chinese Journal of Tropical Crops,2010,31(12):2147-2152. |
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| Authors: | Yin anqi Li hongwu Liu zhiyuan Ban syuuhei Tanabe shouko and Hosoi kungfu |
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| Institution: | Hainan University;Hainan University;Hainan University;Shiga Prefectural University;Kobe University;Fukui Prefectural University |
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| Abstract: | Prorocentrum lima is the main source of diarrhetic shellfish poisoning(DSP), can cause human food poisoning by food chain. The study of application of SYBR Green real-time fluorescence quantitative PCR technique established the method to detect P. lima rapidly, Abstracly, P. lima can be detected by this method with a high sensitivity when the sample contains four cells, and the results showed that during the actual application, measured value was 505±14.72 cells/L(microscopic value was 600 cells/L, error was 15.83%, meeting the plankton investigation required error less than 20% in the ocean survey specification). The detection method is rapid, specific and accurate, can serve as a method for P. lima qualitative and quantitative detection in the marine environmental monitor. |
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