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| 烟草环斑病毒的RT-PCR和IC-RT-PCR检测方法研究 | |
| 引用本文： | 杨翠云,曹洁,于翠,高焕利,沈禹飞,代光辉.烟草环斑病毒的RT-PCR和IC-RT-PCR检测方法研究[J].上海农业学报,2007,23(1):83-87. |
| 作者姓名： | 杨翠云 曹洁 于翠 高焕利 沈禹飞 代光辉 |
| 作者单位： | 1. 上海出人境检验检疫局,上海,200135 2. 第二军医大学微生物学教研室,上海,200433 3. 上海交通大学农业与生物学院,上海,201101 |
| 摘 要： | 根据TRSV CP基因的保守序列设计引物,RT-PCR和IC-RT-PCR能从TRSV的2个分离物中分别扩增到与预期大小相同的DNA条带,序列测定和分析表明所扩增序列为TRSV CP基因的部分序列,在系统关系树上与TRSV的其他分离物形成一簇,表明所建立的RT-PCR和IC-RT-PCR检测方法是可靠的。 |
| 关 键 词： | 烟草环斑病毒(TRSV) 检测方法 |
| 文章编号： | 1000-3924（2007）01-83-05 |
| 修稿时间： | 2005-12-272007-01-10 |
Study on the RT-PCR and IC-RT-PCR method of detecting tobacco ringspot virus |
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| YANG Cui-yun,CAO Jie,YU Cui,GAO Huan-li,SHEN Yu-fei,DAI Guang-hui.Study on the RT-PCR and IC-RT-PCR method of detecting tobacco ringspot virus[J].Acta Agriculturae Shanghai,2007,23(1):83-87. | |
| Authors: | YANG Cui-yun CAO Jie YU Cui GAO Huan-li SHEN Yu-fei DAI Guang-hui |
| Institution: | 1.Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China ; 2 Department of Microbiology, No. 2 Military Medical University, Shanghai 200433, China ; 3 School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, China |
| Abstract: | A pair of specific primers were designed according to the conserved sequence of TRSV coat protein gene,and a specific band with an expected size was amplified respectively from the 2 isolates of TRSV by RT-PCR and IC-RT-PCR.The sequence analysis showed that the determined sequence was a partial sequence of the TRSV coat protein gene and formed one branch on the phylogenetic tree with other isolates of TRSV,which indicated that the established RT-PCR and IC-RT-PCR detection method was reliable. |
| Keywords: | RT-PCR IG-RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |