| 利用山新杨萌生枝叶片建立无菌系的研究 |
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| 引用本文： | 赵凌泉,史绍林,徐连峰,应亚琳.利用山新杨萌生枝叶片建立无菌系的研究[J].防护林科技,2006(4):14-16. |
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| 作者姓名： | 赵凌泉 史绍林 徐连峰 应亚琳 |
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| 作者单位： | 黑龙江省森林与环境科学研究院,黑龙江,齐齐哈尔,161005 |
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| 摘 要： | 以山新杨(24年生)优树的2年生萌生枝上的叶片为材料,进行了不同培养基及不同影响因子对山新杨叶片不定芽再生能力影响试验,结果表明:山新杨叶片再生的最适合培养基是1/2MS+BA0.5 mg.L-1+NAA0.1~0.2 mg.L-1,再生频率达100%,叶片培养的最佳取材部位是树枝茎尖展叶开始第1~3片叶;叶片正面向上或向下接种的培养基,对其形成不定芽无显著影响。
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| 关 键 词： | 山新杨 培养基 再生 不定芽 无菌系 |
| 文章编号： | 1005-5215(2006)02-0014-03 |
| 修稿时间： | 2006年1月20日 |
Study on Using Sprouting Leaves of Populus davidiana × P.× bolleana to Establish Aseptic Strain |
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| ZHAO Ling-quan,SHI Shao-lin,XU Lian-feng,YING Ya-lin.Study on Using Sprouting Leaves of Populus davidiana × P.× bolleana to Establish Aseptic Strain[J].Protection Forest Science and Technology,2006(4):14-16. |
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| Authors: | ZHAO Ling-quan SHI Shao-lin XU Lian-feng YING Ya-lin |
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| Abstract: | Taking leaves of 2-year-old stump plant of selected tree of Populus davidiana×P.bolleana(24-year-old) as materials ,tests of different culture medium and affectoi on regeneration capacity influence of adventitious bud of leaves of Populus davidiana×P.bolleana were conducted.Results showed that the optimal culture medium is 1/2MS+BA0.5 mg·L~(-1)+NAA0.1~0.2 mg·L~(-1).Regeneration frequency reaches 100%.The best parts taken from leaves are on leaf wxpansion(from the first leave to the third leave) of stem apex of branches.Culture medium inoculated upward and downward the pressure side of the blade has no significant influence on forming adventitious bud. |
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| Keywords: | Populus davidiana×P bolleana culture medium regeneration adventitious bud aseptic strain |
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