| 骆驼蓬地上部总黄酮提取工艺的优化 |
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| 引用本文： | 张普照,王俊儒,姚伟琴,张义英,彭菊艳.骆驼蓬地上部总黄酮提取工艺的优化[J].西北农业学报,2005,14(5):114-117. |
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| 作者姓名： | 张普照 王俊儒 姚伟琴 张义英 彭菊艳 |
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| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西,杨凌,712100
2. 新疆出入境检验检疫局检验检疫技术中心,乌鲁木齐,830063 |
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| 基金项目： | 教育部留学回国基金项目"骆驼蓬地上部化学成分及其生物活性的研究"(20040101012)部分内容. |
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| 摘 要： | 以骆驼蓬地上部提取液中总黄酮含量为指标,按正交试验设计,比较超声波提取和热回流提取骆驼蓬总黄酮率的高低.试验结果表明无论以茎叶或种子为提取原料,热回流提取法都获得最高的总黄酮提取率.其最佳提取工艺为以茎叶为原料提取,样液比(W/W)15∶1、时间25 min、乙醇浓度75%、次数3;以种子为原料提取,样液比25∶1、时间25 min、乙醇浓度90%、次数1.在实际应用中,有必要将种子和茎叶分开提取,并将2种提取方法有效结合起来.
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| 关 键 词： | 黄酮 正交设计 骆驼蓬 超声波提取 热回流提取 |
| 文章编号： | 1004-1389(2005)05-0114-04 |
| 收稿时间： | 2005/4/12 0:00:00 |
| 修稿时间： | 2005年4月12日 |
Optimization of Extraction Techniques of Flavonoids in the Aboveground of Peganum harmala. L |
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| ZHANG Pu-zhao,WANG Jun-ru,YAO Wei-qin,ZHANG Yi-ying and PENG Ju-yan.Optimization of Extraction Techniques of Flavonoids in the Aboveground of Peganum harmala. L[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(5):114-117. |
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| Authors: | ZHANG Pu-zhao WANG Jun-ru YAO Wei-qin ZHANG Yi-ying and PENG Ju-yan |
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| Institution: | The College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;The College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;Inspection and Quarantine Technictue Centre, Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumyi Xinjiang 830063, China;The College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;The College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | The present paper was to compare the flavonoids content in the extracts from the aboveground of Peganum hurmulu.L between the ultrasonic extraction method and the reflux method using the orthogonal design of expeiriments.The results suggested that it was the reflux method which got the highest flavonoid content in the extracts whenever the seeds or the stems and leaves were used as the samples.When the stem and leaf was used as the sample,the optimal extracting parameters were as the following:the ratio of solvent to sample (W/W)15:1,extracting duration 25 min,solvent 75%ethanol,extracting time thrice.When the seeds were used as the samples,the optimal extracting parameters were as the following:the ratio of solvent to sample (W/W)25:1, extracting duration 25min,solvent 90% ethanol,extracting time once.In the practical use,it is necessary to combine the two extrtacting methods and extract from the seeds,and the stems & leaves respectively. |
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| Keywords: | Flavonoids The orthogonal design teat Pegunum hurmula L Ultrasonic extracting Reflux extracting |
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