| Cry1Ac和sck双价抗虫基因遗传转化甘蔗的研究 |
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| 引用本文： | 吴转娣,吴才文,曾千春,苏火生,刘新龙,姚丽,张敏,刘家勇.Cry1Ac和sck双价抗虫基因遗传转化甘蔗的研究[J].热带作物学报,2014,35(11):2236-2242. |
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| 作者姓名： | 吴转娣 吴才文 曾千春 苏火生 刘新龙 姚丽 张敏 刘家勇 |
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| 作者单位： | 1. 云南省农业科学院甘蔗研究所 云南省甘蔗遗传改良重点实验室 云南开远661699
2. 云南农业大学,云南昆明,650201 |
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| 基金项目： | 国家现代农业产业技术体系专项资金项目（No. CARS-20-1-1）。 |
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| 摘 要： | 将双价抗虫基因,即修饰的苏云金芽孢杆菌毒蛋白基因(Cry1Ac)和修饰的豇豆胰蛋白酶抑制剂基因(sck)导入甘蔗优良品种ROC22,获得潮霉素抗性转化植株95株,35株能检测到外源抗虫基因,获得的13株RT-PCR双基因均呈阳性的甘蔗植株,斑点杂交检测有4株呈阳性。结果表明:外源抗虫基因已导入甘蔗基因组中。
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| 关 键 词： | 甘蔗 根癌农杆菌介导法 Cry1Ac sck 遗传转化 |
Genetic Transformation of Cry1Ac and sck Gene into Sugarcane |
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| WU Zhuandi,WU Caiwen,ZHEN Qianchun,SU Huosheng,LIU Xinlong,YAO Li,ZHANG Min and LIU Jiayong.Genetic Transformation of Cry1Ac and sck Gene into Sugarcane[J].Chinese Journal of Tropical Crops,2014,35(11):2236-2242. |
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| Authors: | WU Zhuandi WU Caiwen ZHEN Qianchun SU Huosheng LIU Xinlong YAO Li ZHANG Min and LIU Jiayong |
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| Institution: | Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Yunnan Agricultural University;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement |
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| Abstract: | |
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| Keywords: | Sugarcane Agrobacterium-mediated Cry1Ac sck Genetic transformation |
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