| 山蒌单芽茎段培养与快繁技术研究 |
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| 引用本文： | 李新,黄东益,成善汉,唐燕琼,刘进平.山蒌单芽茎段培养与快繁技术研究[J].热带作物学报,2014,35(11):2255-2259. |
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| 作者姓名： | 李新 黄东益 成善汉 唐燕琼 刘进平 |
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| 作者单位： | 1. 海南大学农学院 海南大学热带作物种质资源保护与开发利用教育部重点实验室 海南海口570228
2. 海南大学园艺园林学院,海南海口,570228 |
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| 基金项目： | 海南大学地方服务项目“海南特色蔬菜新品种选育与推广利用”；国家自然科学基金项目“海南野生胡椒种质资源遗传多样性分析和保护利用”（No. 31060204）。 |
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| 摘 要： | 以山蒌单芽茎段为外植体,对其进行组织培养和快速繁殖研究。结果表明:山蒌嫩茎最佳消毒方法为70%酒精消毒30 s,再用0.1%升汞溶液消毒9 min;适合腋芽萌发的启动培养基为MS+0.5 mg/L NAA+3 mg/L 6-BA;继代增殖培养最适宜的培养基为MS+0.1 mg/L NAA+3 mg/L 6-BA;试管苗在1/2MS+3 mg/L NAA+0.1-0.5 mg/L6-BA生根较好;山蒌试管苗移栽成活率可以达到95%以上。
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| 关 键 词： | 山蒌 组织培养 快速繁殖 表面消毒 |
Single-node Stem Culture and Rapid Propagation of Piper sarmentosum |
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| LI Xin,HUANG Dongyi,CHENG Shanhan,TANG Yanqiong and LIU Jinping.Single-node Stem Culture and Rapid Propagation of Piper sarmentosum[J].Chinese Journal of Tropical Crops,2014,35(11):2255-2259. |
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| Authors: | LI Xin HUANG Dongyi CHENG Shanhan TANG Yanqiong and LIU Jinping |
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| Institution: | LI Xin;HUANG Dongyi;CHENG Shanhan;TANG Yanqiong;LIU Jinping;Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources (Hainan University),Ministry of Education / College of Agronomy, Hainan University;College of Horticulture, Hainan University; |
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| Abstract: | Tissue culture and rapid propagation of Piper sarmentosum were conducted with single-node stems as the explants. The results showed that the best surface sterilization was achieved by immersing the explants in the solution of 70% alcohol for 30 seconds then in 0.1% HgCl2 solution for 9 minutes. The medium(MS+0.5 mg/L NAA+3 mg/L 6-BA)was suitable for starting culture of P. sarmentosum. Successful shoot proliferation was obtained on MS supplemented with 0.1 mg/L NAA and 3 mg/L 6-BA. The optimal rooting medium was 1/2MS+3 mg/L NAA+0.1-0.5 mg/L 6-BA. |
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| Keywords: | Piper Sarmentosum Tissue culture Rapid propagation Surface sterilization |
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