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| 利用RT-PCR技术扩增绵羊输卵管上皮细胞Ghrelin基因 | |
| 引用本文： | 高甜,温世勇,钱英红,丛珊,李琦,宋锦,曹贵方.利用RT-PCR技术扩增绵羊输卵管上皮细胞Ghrelin基因[J].畜牧与饲料科学,2014(10):7-9. |
| 作者姓名： | 高甜 温世勇 钱英红 丛珊 李琦 宋锦 曹贵方 |
| 作者单位： | 1. 内蒙古农业大学兽医学院，内蒙古 呼和浩特 010018; 内蒙古医科大学，内蒙古 呼和浩特 010110 2. 内蒙古农业大学兽医学院,内蒙古 呼和浩特,010018 3. 内蒙古农牧业科学院,内蒙古 呼和浩特,010031 |
| 摘 要： | 提取绵羊输卵管上皮细胞总RNA,采用Primer 5.0软件设计引物,运用RT-PCR技术扩增Ghrelin基因。结果显示,经测序和序列比对鉴定,该方法成功地扩增到了大小为200 bp的目的基因,表明RT-PCR技术是检测绵羊输卵管上皮细胞中Ghrelin基因的有效手段,可为今后研究不同激素水平下绵羊输卵管组织中Ghrelin基因的表达量变化及进一步体外试验奠定基础。 |
| 关 键 词： | 输卵管上皮细胞 Ghrelin基因 |
Amplification of Ghrelin Gene in Oviduct Epithelial Cells of Sheep by RT-PCR |
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| GAO Tian,WEN Shi-yong,QIAN Ying-hong,CONG Shan,LI Qi,SONG Jin,CAO Gui-fang.Amplification of Ghrelin Gene in Oviduct Epithelial Cells of Sheep by RT-PCR[J].Animal Husbandry and Feed Science,2014(10):7-9. | |
| Authors: | GAO Tian WEN Shi-yong QIAN Ying-hong CONG Shan LI Qi SONG Jin CAO Gui-fang |
| Institution: | GAO Tian, WEN Shi-yong, QIAN Ying-hong, CONG Shan, LI Qi, SONG Jin, CAO Gui-fang ( 1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Inner Mongolia Medical University,Hohhot 010110,China;3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031, China) |
| Abstract: | Total RNA was extracted from oviduct epithelial cells of sheep,primers were designed by Primer 5.0 software,and Ghrelin gene was amplified by RT-PCR. The results showed that 200 bp of aim gene was obtained successfully by RT-PCR, which indicated that RT-PCR technique was an efficient mean to detect expression of Ghrelin gene in oviduct epithelial cells of sheep. The results lay the basis for studying expression change of Ghrelin gene in oviduct tissues of sheep under different hormone levels and further in vitro test. |
| Keywords: | RT-PCR oviduct epithelial cells Ghrelin gene |
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