| 小麦新品种川农16与新材料H461贮藏蛋白及SSR差异分析 |
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| 引用本文： | 谢玥,龙海,侯永翠,郑有良.小麦新品种川农16与新材料H461贮藏蛋白及SSR差异分析[J].西北农业学报,2005,14(6):34-37. |
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| 作者姓名： | 谢玥 龙海 侯永翠 郑有良 |
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| 作者单位： | 四川农业大学小麦研究所,四川,都江堰,611830 |
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| 基金项目： | 高等学校博士学科点专项科研基金(20040626004) |
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| 摘 要： | 应用酸性聚丙烯酰胺凝胶电泳(A-PAGE)、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和简单重复序列(simple sequence repeat,SSR)标记,对穗数型多分蘖小麦品种川农16与大穗型寡分蘖小麦品系H461生化及分子水平上的遗传差异进行了检测.结果表明,川农16与H461间至少有10条醇溶蛋白差异带,其高分子量谷蛋白亚基组成分别为(1,20,5 10)和(2*,7 8,2 12).在小麦21条染色体上的72个SSR位点上共检测到95个等位变异,每一个位点所检测到的等位变异数目为1至2个,平均1.3个;其中,23对SSR引物(31.9%)能够揭示川农16与H461间的差异.
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| 关 键 词： | 小麦 醇溶蛋白 谷蛋白 SSR标记 遗传差异 |
| 文章编号： | 1004-1389(2005)06-0034-04 |
| 收稿时间： | 2005/6/30 0:00:00 |
| 修稿时间： | 2005年6月30日 |
Storage Protein and SSR Variations in Wheat Genotypes Chuannong 16 and H461 with Great Difference on Spikes Per Plant |
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| XIE Yue,LONG Hai,HOU Yong-cui and ZHENG You-liang.Storage Protein and SSR Variations in Wheat Genotypes Chuannong 16 and H461 with Great Difference on Spikes Per Plant[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(6):34-37. |
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| Authors: | XIE Yue LONG Hai HOU Yong-cui and ZHENG You-liang |
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| Institution: | Triticeac Research Institute, Sichuan Agricultural University, Dujiangyan Sichuan 11830, China;Triticeac Research Institute, Sichuan Agricultural University, Dujiangyan Sichuan 11830, China;Triticeac Research Institute, Sichuan Agricultural University, Dujiangyan Sichuan 11830, China;Triticeac Research Institute, Sichuan Agricultural University, Dujiangyan Sichuan 11830, China |
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| Abstract: | Genetic variations of seed storage protein composition and SSR between a wheat cultivar Chuannong 16 with more spikes per plant and a uniculm wheat line H461 were evaluated. The composition of gliadins, analyzed by the acid polyacrylamide gel eletrophoresis (A-PAGE), indicated that 10 bands were polymorphic between Chuannong 16 and H461. High molecular weight glutenin subunit composition of the two genotypes were identified by sodium dodeeyl sulphate-polyacrylamid gel electrophoric (SDS-PAGE), indicating that Chuannong 16 has 1,20,54-10 subunits and H461 has 2 * ,7 8,2 12 subunits. A total of 95 alleles were detected on 72 SSR loci. On each SSR locus, 1 to 2 alleles were detected, with the mean of 1. 3 alleles. Twenty-three out of 72 SSR markers (31. 9%) were polymorphic between the two genotypes. |
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| Keywords: | Wheat Gliadin Glutenin SSR markers Genetic variation |
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