| 甘蓝小孢子植株倍性简易鉴定方法研究 |
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| 引用本文： | 邓耀华,熊艺,周国林,汪爱华.甘蓝小孢子植株倍性简易鉴定方法研究[J].中国蔬菜,2009,1(18):46-50. |
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| 作者姓名： | 邓耀华 熊艺 周国林 汪爱华 |
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| 作者单位： | 1.武汉市蔬菜科学研究所，湖北武汉430065;2.武汉工程大学,湖北武汉430073 |
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| 摘 要： | 以编号为12、22的甘蓝材料（F1）经小孢子培养得到的植株为试材，先利用流式细胞仪对供试材料进行倍性鉴定，再利用普通光学显微镜观察已通过倍性鉴定的双单倍体与单倍体的气孔保卫细胞，以测定其大小与叶绿体数。结果表明：气孔保卫细胞叶绿体数与甘蓝倍性高低呈正比关系，甘蓝双单倍体气孔保卫细胞叶绿体数变异幅度为12~17，单倍体气孔保卫细胞叶绿体数的变异幅度为6~10；同一材料双单倍体与单倍体气孔保卫细胞叶绿体数的比值≈2，两者数值差异非常显著直观，易于辨别。因此观测气孔保卫细胞叶绿体数是鉴定甘蓝小孢子植株倍性的简易有效手段。
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| 关 键 词： | 甘蓝 小孢子植株 倍性鉴定 气孔保卫细胞 叶绿体数 |
| 收稿时间： | 2008-12-12; |
Studies on Facility Identification Method for Ploidy of Cabbage Microspore Plant |
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| DENG Yao-hua,XIONG Yi,ZHOU Guo-lin,WANG Ai-hua.Studies on Facility Identification Method for Ploidy of Cabbage Microspore Plant[J].China Vegetables,2009,1(18):46-50. |
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| Authors: | DENG Yao-hua XIONG Yi ZHOU Guo-lin WANG Ai-hua |
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| Institution: | 1.Wuhan Vegetable Research Institute,Wuhan 430065,Hubei,China;2.Wuhan University of Technology,Wuhan 430073,Hubei,China |
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| Abstract: | The cultivars obtained by microspore culture in cabbage of two F1 cultivars No.12 and No.22 were studied.Firstly,ploidys of the two F1 cultivars were detected by using flow cytometry.Secondly,the size of stomata protective cells was observed and the chloraplast number of doubled haploid cultivars and haploid cultivars with ploidy were counted and identified.The results show that there is a positive relationship between the chloraplast number of stomata protective cells and ploidy of cabbage.The chloraplast numbers of stomata protective cells in doubled haploid cultivars vary from 12 to 17 and that in haploid cultivars vary from 6 to 10.Thus the ratio of the chloraplast number of stomata protective cells in doubled haploid cultivars to that in haploid cultivars is about 2,and there is big difference between the chloraplast number of stomata protective cells in doubled haploid cultivars and of haploid cultivars.So observing the chloraplast number of stomata protective cells could be an easy and effective mean to identify ploidy of cabbage. |
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