| 河西走廊牛源A型多杀性巴氏杆菌灭活疫苗的制备及临床效果检测观察 |
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| 引用本文： | 贺奋义,魏玉明,郭慧琳,孟林明,何彦春,张文波.河西走廊牛源A型多杀性巴氏杆菌灭活疫苗的制备及临床效果检测观察[J].畜牧兽医杂志,2014(3):17-20. |
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| 作者姓名： | 贺奋义 魏玉明 郭慧琳 孟林明 何彦春 张文波 |
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| 作者单位： | 甘肃省动物疫病预防控制中心;张掖市动物疫病预防控制中心 |
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| 摘 要： | 根据牛源荚膜血清A型多杀性巴氏杆菌的培养特性,本研究采用脑心浸液肉汤（BHI）培养基静止培养24 h,每2h震荡一次的方法进行培养,将培养的A型多杀性巴氏杆菌灭活后制成A型多杀性巴氏杆菌灭活疫苗,免疫小白鼠和日本大耳白家兔.结果显示：A型灭活苗的保护率为100％;将制备的灭活疫苗进行肉牛安全性试验,均未发现过敏反应等异常情况;在河西走廊嘉峪关、酒泉、张掖、金昌和武威5市应用对网牛春（2～3月）、秋（10～11月）两季分别免疫注射制备的A型多杀性巴氏杆菌灭活疫苗,剂量为体重100 kg以上6 mL/头,100 kg以下4 mL/头的方法进行了较大规模的临床效果观察,免疫牛均未发现不良反应,实验组牛巴氏杆菌病发病、死亡率分别分别比对照组低2.4、1.99个百分点,差异极显著（p＜0.01）.
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| 关 键 词： | 多杀性巴氏杆菌 荚膜血清A型 灭活疫苗制备 效果观察 |
Bovine A Pasteurella Vaccine Preparation and Observation of Clinical Effect Detection in Gansu Corridor |
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| HE Fen-yi;WEI Yu-ming;GUO Hui-lin;MENG Lin-ming;HE Yan-chun;ZHANG Wen-bo.Bovine A Pasteurella Vaccine Preparation and Observation of Clinical Effect Detection in Gansu Corridor[J].Journal of Animal Science and Veterinary Medicine,2014(3):17-20. |
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| Authors: | HE Fen-yi;WEI Yu-ming;GUO Hui-lin;MENG Lin-ming;HE Yan-chun;ZHANG Wen-bo |
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| Institution: | HE Fen-yi;WEI Yu-ming;GUO Hui-lin;MENG Lin-ming;HE Yan-chun;ZHANG Wen-bo;Gansu Animal Disease Prevention and Control Center;Zhangye City Animal Disease Prevention and Control Center; |
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| Abstract: | According to the characteristics of Pasteurella bovine type A, this study aimed to culture this kind of cells in BHI medium for 24h, shocking each 2h, the cultured type A Pasteurella inactivated vaccine made from inactivated Pasteurella multo- cida type A, immune mice and Japanese rabbits. The results showed that vaccine protection rate was 100%. Beef safety trials found that no allergic reactions. During the spring （February-March） and autumn （October-November） on Corridor 5 City （Jia- yuguan, Jiuquan, Zhangye, Jinchang and Wuwei） type A Pasteurella cattle injectable inactivated vaccine （weighing more than 100kg 6ml / head, 100kg less 4ml / head）, the clinical effects were observed, no adverse reactions were found for vaccination of cattle, the experimental group bovine Pasteurella disease than the control group 2.4% lower than the control group mortality rate（ 1.99%）, the difference was significant （P〈0.01）. |
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| Keywords: | Pasteurella multocida~ capsular serum type A~ inactivated vaccine~ effect |
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