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一株脱氮细菌的分离鉴定及脱氮活性检测
引用本文:高存川,张晓慧,徐春厚.一株脱氮细菌的分离鉴定及脱氮活性检测[J].广东畜牧兽医科技,2012,37(3):39-41.
作者姓名:高存川  张晓慧  徐春厚
作者单位:广东海洋大学动物科学系,广东湛江,524088
基金项目:基金项目:广东省科技计划项目
摘    要:利用硅胶平板从淤泥中分离到一株脱氮茵FX7,该菌株在好氧条件下具有降解亚硝态氮的能力。亚硝态氮初始浓度为2500mg/L时,该菌株在48h内亚硝态氮降解率为45%。通过形态学特征、生理生化特性及16SrDNA同源性比较对菌株FX7进行鉴定,初步判断其为假单胞菌(Pseudomonassp)。Pb^2+、Hg^2+和Cr^2+对菌株FX7的亚硝态氮降解活性有很强的抑制作用,而低浓度的Zn^2+和Fe^3+对其活性有一定的激活作用。

关 键 词:益生菌  生物脱氮  分离鉴定

Isolation and identification of a denitrogenation microorganism and its nitrogen removal activity
solation and identification of a denitrogenation microorganism and its nitrogen removal activity Gao Cunchuan,Zhang Xiaohui,Xu Chunhou.Isolation and identification of a denitrogenation microorganism and its nitrogen removal activity[J].Guangdong Journal of Animal and Veterinary Science,2012,37(3):39-41.
Authors:solation and identification of a denitrogenation microorganism and its nitrogen removal activity Gao Cunchuan  Zhang Xiaohui  Xu Chunhou
Institution:(Animal Science Department,Guangdong Ocean University,Zhanjiang 524088,China)
Abstract:A denitrogenation microorganism FX7 was isolated with silicate plate from soil.The experiment results showed that under aerobic conditions the reduction efficiency within 48 hours was up to 45% with initial nitrite nitrogen concentration of 2500 mg/L.On the basis of its physiological and molecular properties,strain FX7 was identified as Pseudomonas sp.Though the nitrite nitrogen removal ability of strain FX7 was strongly inhibited by ions Pb2+,Hg2+and Cr7+,the nitrite nitrogen removal ability was activated by ions of Zn2+ and Fe3+ with low concentration.
Keywords:Probiotics  Biological nitrogen removal  Isolation and identification
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