| 秋水仙素诱导三种紫薇多倍体的研究 |
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| 引用本文： | 童俊,叶要妹,冯彪,袁玮.秋水仙素诱导三种紫薇多倍体的研究[J].园艺学报,2009,36(1):127-132. |
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| 作者姓名： | 童俊 叶要妹 冯彪 袁玮 |
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| 作者单位： | （华中农业大学园艺林学学院，园艺植物生物学教育部重点实验室，武汉430070；武汉市林业果树科学研究所，武汉 430075） |
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| 基金项目： | 华中农业大学大学生科技创新基金 |
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| 摘 要： | 研究了秋水仙素处理浓度和时间对3种紫薇幼苗染色体加倍的诱导效应，通过染色体计数、流式细胞仪以及形态观察等方法进行了倍性鉴定。结果表明：秋水仙素诱导3种紫薇均得到了四倍体,0.5%～0.8%的秋水仙素处理紫薇和银薇48～96 h的变异率较高，其中以0.5%秋水仙素处理紫薇72 h的变异率最高，达54.17%。根尖染色体压片表明四倍体染色体数为：2n=4x=96，二倍体对照为：2n=2x=48。四倍体表现出叶片长、宽、厚均增大，气孔密度减小，气孔保卫细胞增大，保卫细胞内叶绿体数增加，花粉粒直径增大，花径、花瓣长、花瓣基部爪长均较二倍体明显增加，单朵花观赏价值大大提高。
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| 关 键 词： | 紫薇 秋水仙素 多倍体 诱导 鉴定 |
| 收稿时间： | 2008-08-24 |
Colchicines Induced Polyploid Plants and Their Identification in Three Species of Lagerstroemia indica |
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| TONG Jun,YE Yao-mei,FENG Biao,YUAN Wei.Colchicines Induced Polyploid Plants and Their Identification in Three Species of Lagerstroemia indica[J].Acta Horticulturae Sinica,2009,36(1):127-132. |
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| Authors: | TONG Jun YE Yao-mei FENG Biao YUAN Wei |
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| Institution: | (Key Laboratory of Horticultural Plant Biology, Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070, China; Wuhan Forestry & Fruits Research Institute, Wuhan 430075,China) |
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| Abstract: | The effects of colchicine concentration and treatment time duration on the apical meristems of the seedlings in three species of Lagerstroemia indica have been conducted in this study, and the ploidy level was identified by morphological trait, chromosome number and flow cytometry techniques. The results showed that treated L.indica or L.indica var.alba with 0.5%-0.8% colchicine for 48-96 h lead to a high mutation rate, and the highest mutation rate (up to 54.17%) was achieved in the L.indica by treatment of 0.5% colchicine for 72 h. All the three tested species of L.indica have obtained a number of tetraploidy plants. The choromosome number of mutated plants was 2n=4x=96, while that of the control plants was 2n=2x=48. The tetraploid plants exibited some morphological and cytological variations, including larger and thicker leaves, darker leaf color, larger stomata, fewer stomata per unit area, more chloroplasts in stomata guard cell and longer pollen diameter. The ornamental value of single flower had also been greatly improved by increasing of flower diameter, petals and basal claw length. |
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| Keywords: | Lagerstroemia indica Lagerstroemia indica colchicine polyploid induction identification |
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