| 农杆菌蘸花法侵染拟南芥的研究 |
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| 引用本文： | 许红梅,张立军,刘淳.农杆菌蘸花法侵染拟南芥的研究[J].北方园艺,2010(14):143-146. |
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| 作者姓名： | 许红梅 张立军 刘淳 |
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| 作者单位： | 沈阳农业大学,辽宁,沈阳,110161;黑龙江农业职业技术学院,黑龙江,佳木斯,154007;沈阳农业大学,辽宁,沈阳,110161 |
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| 摘 要： | 为探索拟南芥转化法的广泛性,使其能转化其它植物,研究了转化试验中的一些参数.结果表明:农杆菌渗透转化拟南芥成功的3个关键因素是:适当的植株发育时期,即花蕾期;5%的蔗糖;0.05%的Silwet L-77.该种方法可适用于多种生态型的拟南芥植物转化,便于快速获得带有T-DNA标记的植株,并为植物的转基因技术提供一定的参考依据.
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| 关 键 词： | 农杆菌 蘸花法 侵染 拟南芥 |
Study of Infection Arabidopsis thaliana by Agrobacterium Dipping Flower Method |
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| XU Hong-mei,ZHANG Li-jun,LIU Chun.Study of Infection Arabidopsis thaliana by Agrobacterium Dipping Flower Method[J].Northern Horticulture,2010(14):143-146. |
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| Authors: | XU Hong-mei ZHANG Li-jun LIU Chun |
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| Institution: | XU Hong-mei1,2,ZHANG Li-jun1,LIU Chun1(1.Shenyang Agricultural University,Shenyang,Liaoning 110161,2.Heilongjiang Agricultural Vocational and Technical College,Jiamusi,Heilongjiang 154007) |
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| Abstract: | To improve the transformation method of Arabidopsis thaliana very widely and to gain insight that may facilitate transformation of other plant species,we sought to test a number of parameters in the transformation method.The results showed that the three main requirements for successful Agrobacterium-mediated transformation of Arabidopsis thaliana were correct plant developmental stage(floral buds stage) and 5%sucrose plus 0.05%Silwet L-77.The method was very successful for transformation of multipleArabido... |
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| Keywords: | Agrobacterium floral dip inoculation Arabidopsis thaliana |
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