| 不同蛋白水平下添加抗菌肽对赤点石斑鱼影响的研究 |
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| 引用本文： | 蔡云川,赵书燕,林黑着,黄忠,周传朋,马志洲.不同蛋白水平下添加抗菌肽对赤点石斑鱼影响的研究[J].上海海洋大学学报,2017,26(3):383-391. |
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| 作者姓名： | 蔡云川 赵书燕 林黑着 黄忠 周传朋 马志洲 |
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| 作者单位： | 广东省海洋与渔业技术推广总站, 广东 广州 510220,中国水产科学研究院南海水产研究所 农业部南海渔业资源开发利用重点实验室, 广东 广州 510300;上海海洋大学 水产与生命学院, 上海 201306,中国水产科学研究院南海水产研究所 农业部南海渔业资源开发利用重点实验室, 广东 广州 510300,中国水产科学研究院南海水产研究所 农业部南海渔业资源开发利用重点实验室, 广东 广州 510300,中国水产科学研究院南海水产研究所 农业部南海渔业资源开发利用重点实验室, 广东 广州 510300,广东省海洋与渔业技术推广总站, 广东 广州 510220 |
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| 基金项目： | 公益性行业（农业）科研专项经费项目（20140311）；广东省海洋渔业科技推广专项项目（B201000B14，B201101B04，B201201B09） |
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| 摘 要： | 评估了不同饲料蛋白水平(44%,48%,52%)和抗菌肽浓度(0.01%,0.02%)对赤点石斑鱼生长性能、血清生化、消化酶和抗氧化能力的影响。实验组分别为:D1(44%,0.01%),D2(48%,0.01%),D3(52%,0.01%),D4(44%,0.02%),D5(48%,0.02%),D6(52%,0.02%)。每组3个平行,养殖8周结果如下:一定范围内,增重率、特定生长率和饲料转化率随蛋白水平和抗菌肽浓度增加,D5组显著高于D1组(P0.05)。形态学指标,D6组肝体比(HSI)、脏体比(VSI)和肠系膜脂肪(IPF)显著低于D5组(P0.05)。同一蛋白水平下增加抗菌肽浓度可降低肌肉粗脂肪含量,D4组显著低于D1组(P0.05)。血清总蛋白、白蛋白含量随蛋白水平和抗菌肽浓度的增加而升高(P0.05),D3和D5组谷丙转氨酶、谷草转氨酶和血糖显著低于其他组(P0.05)。D5组胰蛋白酶高于其他组(P0.05),D1组过氧化物酶显著低于其他组(P0.05)。随着抗菌肽浓度增加,胃蛋白酶、过氧化物酶、过氧化氢酶活性增加,且丙二醛含量降低(P0.05)。实验结果表明,48%蛋白水平下添加0.02%抗菌肽可以提高赤点石斑鱼生长性能,增强消化酶活性和抗氧化能力,降低饲料系数。
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| 关 键 词： | 石斑鱼 抗菌肽 蛋白 生长 血清生化 抗氧化能力 |
| 收稿时间： | 2016/8/3 0:00:00 |
| 修稿时间： | 2017/1/13 0:00:00 |
Effect of antibacterial peptides supplementation in different protein level diets on grouper (Epinephelus akaara) |
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| CAI Yunchuan,ZHAO Shuyan,LIN Heizhao,HUANG Zhong,ZHOU Chuanpeng and MA Zhizhou.Effect of antibacterial peptides supplementation in different protein level diets on grouper (Epinephelus akaara)[J].Journal of Shanghai Ocean University,2017,26(3):383-391. |
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| Authors: | CAI Yunchuan ZHAO Shuyan LIN Heizhao HUANG Zhong ZHOU Chuanpeng and MA Zhizhou |
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| Institution: | Guangdong Ocean and Fishery Technology Extension Center, Guangzhou 510220, Guangdong, China,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China;School of Life Science and Technology, Shanghai Ocean University, Shanghai 201306, China,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China and Guangdong Ocean and Fishery Technology Extension Center, Guangzhou 510220, Guangdong, China |
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| Abstract: | This experiment was conducted to assess the effects of dietary protein (44%,48% and 52%) and antibacterial peptide (0.01% and 0.02%) on growth performance, serum biochemistry, digestive enzyme and antioxidant abilities of grouper (Epinephelus akaara). The experimental diets were named as:D1 (44%, 0.01%), D2 (48%, 0.01%), D3 (52%, 0.01%), D4 (44%, 0.02%), D5 (48%, 0.02%) and D6 (52%, 0.02%). Each diet was fed to 3 replicates and the experiment lasted for 8 weeks. The results show as follows:In certain range, Weight gain (WG), specific growth rate (SGR) and feed conversion ratio increased with protein level and ABP concentration increasing, and D5 was significantly higher than D1 (P<0.05). The morphological indexes, Hepatosomatic index (HSI), Viscerosomatic index (VSI) and Intraperitoneal fat index (IPF) of D6 were significantly lower than D5 (P<0.05). Crude fat content of fish fed diet D4 was significantly lower than D1 (P<0.05). Serum total protein (TP) and albumin (ALB) increased with protein and ABP increasing (P<0.05), glutamic-pyruvic transaminase (ALT), glutamic oxalacetic transaminase (AST) and glucose (GLU) were lower in D3 and D5 (P<0.05). Trypsase of D5 was higher than other groups (P<0.05), and Peroxidase (POD) of D1 was significantly lower than other groups (P<0.05). ABP could increase Pepsin, Catalase (CAT), POD and decrease Malondialdehyde (MDA) content (P>0.05). The result shows 0.02% ABP supplementation at 48% protein level can improve growth, increase digestive enzyme activities and antioxidant abilities, and decrease FCR. |
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| Keywords: | grouper antibacterial peptides protein growth serum biochemical indices antioxidant abilities |
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