| 水稻抗白叶枯病基因Xa23的PCR分子标记定位及辅助选择 |
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| 引用本文： | 潘海军,王春连,赵开军,章琦,樊颖伦,周少川,朱立煌.水稻抗白叶枯病基因Xa23的PCR分子标记定位及辅助选择[J].作物学报,2003,29(4):501-507. |
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| 作者姓名： | 潘海军 王春连 赵开军 章琦 樊颖伦 周少川 朱立煌 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所,农业部作物遗传育种重点开放实验室,北京,100081 |
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| 基金项目： | 国家植物转基因及其产业化专项 (J0 0 A 0 0 2 )，国家自然科学基金项目 ( 3 9970 45 2 )，国家“863”计划项目 ( 2 0 0 1AA2 2 2 15 1) |
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| 摘 要： | 用Xa23的近等基因系CBB23及其感病轮回亲本JG30构建了包含2562个单株的F2作图群体.通过分析571个感病单株,找到2个新的与Xa23基因连锁的SSR标记RM187和RM206,它们与Xa23之间的遗传图距分别为7.1 cM和1.9 cM.通过筛选1200个RAPD引物,获得2个与Xa23基因连锁的RAPD标记RpdH5和RpdS1184,与Xa23之间的遗传图距分别为7.0 cM和7.6 cM
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| 关 键 词： | Xa23 水稻白叶枯病 SSR RAPD MAS |
| 收稿时间： | 2002-06-26 |
| 修稿时间： | 2002年6月26日 |
Molecular Mapping by PCR-based Markers and Marker-assisted Selection of Xa23, a Bacterial Blight Resistance Gene in Rice |
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| Pan Haijun,Wang Chunlian,Zhao Kaijun,Zhang Qi,Fan Yinglun,Zhou Shaochuan,Zhu Lihuang.Molecular Mapping by PCR-based Markers and Marker-assisted Selection of Xa23, a Bacterial Blight Resistance Gene in Rice[J].Acta Agronomica Sinica,2003,29(4):501-507. |
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| Authors: | Pan Haijun Wang Chunlian Zhao Kaijun Zhang Qi Fan Yinglun Zhou Shaochuan Zhu Lihuang |
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| Abstract: | A F 2 population, with 2562 individuals, of JG30/CBB23 was constructed for molecular mapping of the bacterial blight resistance gene Xa 23 in rice. After screening 1200 random amplified polymorphic DNA (RAPD) primers, we found RAPD markers, RpdH5 and RpdS1184, were linked to the Xa 23 gene, with genetic distance of 7.0 cM and 7.6 cM, respectively. Two simple sequence repeats (SSR) markers, RM187 and RM 206, were also found to be linked to the Xa 23 gene, the genetic distance were 7.1 cM and 1.9 cM, respectively. The marker assisted selection (MAS) efficiency of RpdS 1184, RM 206 and RpdH5 was evaluated using two true breeding F 2 populations. The results showed that the MAS efficience of RpdH5 and RpdS1184, in population Fengfu23, were 91.0% and 87.3%, respectively, but the MAS efficiency reached as high as 99% when these two markers were co used. For population Luyou 23, the MAS efficiency of RpdH5, RpdS1184 and RM 206 were 77.1%, 81.1% and 80.8%, respectively. When RpdH5 and RpdS1184 were co used, the MAS efficiency was 90.3%; when RpdH5 and RM 206 were co used, the MAS efficiency was 91.3%; when RpdH5, RM 206 and RpdS1184 were co used, the MAS efficiency was 90.8%. |
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| Keywords: | Xa 23 Rice bacterial blight SSR RAPD MAS |
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