New severe strains of Melon necrotic spot virus: symptomatology and sequencing |
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| Authors: | C Kubo E Nakazono-Nagaoka K Hagiwara H Kajihara S Takeuchi K Matsuo T U Ichiki T Omura † |
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| Institution: | National Agricultural Research Center, Tsukuba, Ibaraki, 305-8666;;Chiba Prefectural Agriculture Research Center, Chiba, 266-0006;;Yamaguchi Prefectural Agricultural Experiment Station, Yamaguchi, 753-0214;;Kochi Agricultural Research Center, Kochi, 783-0023;and;Nagasaki Agricultural and Forestry Experiment Station, Nagasaki, 854-0063, Japan |
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| Abstract: | New strains of Melon necrotic spot virus (MNSV), designated MNSV-YS and MNSV-KS, caused much more severe growth retardation on melon plants than MNSV-NH, which was previously reported as the most severe strain of MNSV in Japan. MNSV-YS spread much more quickly than MNSV-NH in infected plants, and induced more severe growth retardation, even though the appearance of necrotic lesions on inoculated cotyledons was much slower. MNSV-KS had properties intermediate between those of the other two strains. The results suggest that faster-spreading strains can multiply more rapidly as a result of lower levels of activity in inducing necrotic lesions in melon plants. The complete sequences of MNSV-YS and MNSV-KS were determined, and an RT–PCR–RFLP method based on these sequences was successfully developed to detect and discriminate between the three strains. |
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| Keywords: | genome sequencing MNSV RT–PCR–RFLP tissue immunoblot virus strains |
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