| 枯草杆菌的淀粉酶基因在大肠杆菌中的表达 |
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| 引用本文： | 尹清强,韩彪,郑秋红,康相涛.枯草杆菌的淀粉酶基因在大肠杆菌中的表达[J].畜牧与兽医,2005,37(6):7-9. |
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| 作者姓名： | 尹清强 韩彪 郑秋红 康相涛 |
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| 作者单位： | 河南农业大学牧医工程学院,河南,郑州,450002 |
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| 摘 要： | 根据枯草杆菌(Bacillus subtilis)基因文库中的淀粉酶基因的碱基序列,设计引物。以枯草杆菌菌体DNA为模板,利用PCR扩增出分子量大约为2.3 kb的淀粉酶基因片段。借助于核酸内切酶和连接酶把该基因植入到pHMSXD1质粒中,构建pHMSXD2质粒载体。通过高压电击处理把载体植入到大肠杆菌(JM10)中并成功表达。该株大肠杆菌在酶基因表达后,其淀粉酶活力达到1.037 6 U/mL(LB培养液)和1.361 0 U/mL(矿物元素培养液),分别比原始的枯草杆菌的淀粉酶活力提高了46倍和286倍。
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| 关 键 词： | 淀粉酶基因 转基因 大肠杆菌 枯草杆菌 |
| 文章编号： | 0529-5130（2005）06-0007-03 |
| 收稿时间： | 10 19 2004 12:00AM |
| 修稿时间： | 2004年10月19 |
Expression of amylase gene from Bacillus subtilis in transformed Escherichia coli |
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| YIN Qing-qiang,Han Biao,ZHENG Qiu-hong,HANG Xiang-tao.Expression of amylase gene from Bacillus subtilis in transformed Escherichia coli[J].Animal Husbandry & Veterinary Medicine,2005,37(6):7-9. |
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| Authors: | YIN Qing-qiang Han Biao ZHENG Qiu-hong HANG Xiang-tao |
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| Abstract: | Amylase gene (2.3 kb) form Bacillus subtilis was amplified by polymerase chain reaction (PCR). After the amylase genes were connected with a plasmid (pHMSXD1) and transformed into Escherichia coli (E.coli) by electroporation,the transformed E.coli could express and secret amylase. Compared with the original B.subtilis, the amylase activitives from transformed E.coli were 1.037 6 U/mL in LB medium and 1.361 0 U/mL in a special mineral medium, which were increased by 46 and 286 folds, respectively. |
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| Keywords: | amylase gene transformation Escherichia coli Bacillus subtilis |
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