| 杜鹃组织培养研究进展 |
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| 引用本文： | 邱璐,梁晓华,黄静,陈凯,周银燕,杨启国,郑才智.杜鹃组织培养研究进展[J].安徽农业科学,2005,33(9):1717-1719. |
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| 作者姓名： | 邱璐 梁晓华 黄静 陈凯 周银燕 杨启国 郑才智 |
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| 作者单位： | 楚雄师范学院,云南,楚雄,675000 |
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| 摘 要： | 杜鹃组织培养以茎尖、带腋芽的幼茎、幼叶、花雷、种子等作外植体;以浓度0.1%HgCl2消毒5~8 min,或浓度0.2%HgCl2消毒2~3min;以Read、Mccown、Anderson1、/4 MS1、/10MS、MS、改良MS、ER等为基本培养基;以ZT2.0~5.0 mg/L或2ip10~15 mg/L或KT 2.0~5.0mg/L,配合NAA或IAA 0.05~0.5 mg/L诱导不定芽及愈伤组织;以IBA或NAA为0.5~1.0 mg/L+活性炭0~0.3%诱导根形成;以浓度3%的蔗糖作碳源;以AC(活性炭)0.1%、PVP(聚乙烯吡咯烷酮)100 mg/L控制褐化;pH值为5.0~5.4;光照强度为1 500~2 500 lx,光照时间为16 h/d,黑暗8 h/d,培养温度(25+2)℃。
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| 关 键 词： | 杜鹃 组织培养 外植体 消毒剂 培养基 激素 外界条件 |
| 文章编号： | 0517-6600（2005）09-1717-03 |
| 收稿时间： | 07 8 2005 12:00AM |
| 修稿时间： | 2005-07-08 |
Study Advance in Rhododendron Tissue Culture |
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| Qiu Lu;Liang XiaoHua;Huang Jing;Chen Kai;Zhou YinYan;Yang QiGuo;Zheng CaiZhi.Study Advance in Rhododendron Tissue Culture[J].Journal of Anhui Agricultural Sciences,2005,33(9):1717-1719. |
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| Authors: | Qiu Lu;Liang XiaoHua;Huang Jing;Chen Kai;Zhou YinYan;Yang QiGuo;Zheng CaiZhi |
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| Abstract: | The Rhododendron shoot tip,cladium,delicate leaf,alabastrum and seed are usually used as explants in tissue culture.0.1% HgCl_2 or(0.2%) HgCl_2 was the best disinfecting liquid.The best medium was Read,Mccown,Anderson,1/4 MS,1/10MS,MS and ER,and the best optimal hormone component and concentration were 2.0～5.0?mg/L ZT,10～15?mg/L 2ip or 2.0～5.0?mg/L KT and 0.05～0.5?mg/L NAA or IAA in induction culture for callus and adventitious bud.0.5～1.0?mg/L IBA or NAA and 0～0.3% AC was the best in root formation.3%sugar was the best carbon origin.0.1%AC and 100?mg/L?PVP,pH value=5.0～5.4 1?500～2?500?lx for 16?h/d and(25+2)℃ was beneficial to healthy development. |
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| Keywords: | Rhododendron Tissue culture Explants Disinfecting liquid Medium Hormone Culture condition |
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