| 生防放线菌HNA30的固体发酵条件 |
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| 引用本文： | 刘欢,雷平,郭照辉,刘宇波,曾奥,肖蓉,胡展.生防放线菌HNA30的固体发酵条件[J].贵州农业科学,2017,45(4). |
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| 作者姓名： | 刘欢 雷平 郭照辉 刘宇波 曾奥 肖蓉 胡展 |
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| 作者单位： | 湖南省微生物研究院,湖南长沙,410009 |
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| 基金项目： | 湖南省自然科学基金项目“内生放线菌OsiSh-2拮抗水稻稻瘟病菌的活性成分及作用机理研究”，湖南省科技计划项目“利用拮抗微生物防治水稻稻瘟病的研究和应用” |
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| 摘 要： | 为生防放线菌HNA30的生产应用提供依据,通过单因素试验和正交试验对其固体发酵条件进行优化。结果表明:固体发酵的最佳基质为麦麸,最佳碳源为淀粉,最佳氮源为酵母提取物;菌株HNA30固体发酵的最佳条件为淀粉50mg/g、酵母提取物30mg/g、初始含水量400mg/g、接种量0.35mL/g、培养时间168h;该条件下,HNA30的固体发酵产孢量达1.07×10~(10) cfu/g。
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| 关 键 词： | 固体发酵 优化 HNA30菌株 放线菌 |
Solid Fermentation Conditions of Biocontrol Actinomycete HNA30 |
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| LIU Huan,LEI Ping,GUO Zhaohui,LIU Yubo,ZENG Ao,XIAO Rong,HU Zhan.Solid Fermentation Conditions of Biocontrol Actinomycete HNA30[J].Guizhou Agricultural Sciences,2017,45(4). |
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| Authors: | LIU Huan LEI Ping GUO Zhaohui LIU Yubo ZENG Ao XIAO Rong HU Zhan |
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| Abstract: | In order to provide references for the production application of biocontrol actinomycete HNA30,a single factor experiment and an orthogonal experiment was conducted to optimize the solid fermentation conditions.Results:The optimal substrate,carbon source and nitrogen source were wheat bran,starch and yeast extract respectively.Besides,the optimum fermentation conditions were detailed as follows:starch 50 mg/g,yeast extract 30 mg/g,initial moisture content 400 mg/g,inoculums size 0.35 mL/g,incubation time 168 h;The spore-bearing yield of this strain has attained up to 1.07× 1010 cfu/g under these conditions. |
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| Keywords: | solid fermentation optimization strain HNA30 biocontrol actinomycete |
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