| 枯草芽孢杆菌HAS中几丁质酶基因的克隆分析 |
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| 引用本文： | 熊国如,赵更峰,张树珍,蔡文伟,伍苏然,杨本鹏.枯草芽孢杆菌HAS中几丁质酶基因的克隆分析[J].贵州农业科学,2017,45(2). |
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| 作者姓名： | 熊国如 赵更峰 张树珍 蔡文伟 伍苏然 杨本鹏 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所/甘蔗研究中心,海南海口,571101;2. 海南医学院理学院,海南海口,571101 |
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| 基金项目： | 国家自然科学基金面上项目“甘蔗黑穗病拮抗菌株HAS抑菌机理研究”，海南省自然科学基金项目“甘蔗黑穗病拮抗菌株HAS防病机制研究”，现代农业产业技术体系建设专项“甘蔗植保岗位科学家经费” |
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| 摘 要： | 为揭示枯草芽孢杆菌菌株HAS对甘蔗黑穗病菌抑制作用的机理,选取对多种病原真菌有抑制作用的几丁质酶进行克隆与分析。结果表明:菌株HAS基因组中含有1个ORF全长834bp的几丁质酶编码序列,其具有编码278个氨基酸的几丁质酶蛋白,该序列同GenBank上登录的几丁质酶蛋白(Bacillus subtilis subsp.subtilis str.168)(ref|NP_390566.1)的同源性最高,达98%。推测该几丁质酶在菌株HAS抑制甘蔗黑穗病菌过程中可能起关键作用。
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| 关 键 词： | 枯草芽孢杆菌HAS 黑穗病菌 抗菌蛋白 几丁质酶基因 |
Cloning and Analysis of Chitinase Gene in Bacillus subtilis HAS |
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| XIONG Guoru,ZHANG Gengfeng,ZHANG Shuzhen,CAI Wenwei,WU Suran,YANG Benpeng.Cloning and Analysis of Chitinase Gene in Bacillus subtilis HAS[J].Guizhou Agricultural Sciences,2017,45(2). |
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| Authors: | XIONG Guoru ZHANG Gengfeng ZHANG Shuzhen CAI Wenwei WU Suran YANG Benpeng |
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| Abstract: | The chitinase with an inhibition effect on various pathogenic fungi was cloned and analyzed to reveal the inhibition mechanism of B.subtilis HAS against sugarcane S.scitaminea.Results: The genome of HAS strain contains a chitinase coding sequence with 834 bp and the chitinase coding sequence has chitinase protein with 278 animo acids.The homology between the chitinase coding sequence and B.subtilis subsp.subtilis str.168)(ref| NP_390566.1)listed in GenBank is up to 98％.In conclusion, the chitinase in B.subtilis HAS strain may play a vital role to inhibit sugarcane S.scitaminea. |
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| Keywords: | Bacillus subtilis HAS Sporisorium scitaminea antifungal protein chitinase gene |
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