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| 法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达 | |
| 引用本文： | 宋海涛,张红,王丽,段艳华,乔松林,李学伍,赵光辉,张改平.法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达[J].河南农业科学,2007(3):109-111. |
| 作者姓名： | 宋海涛 张红 王丽 段艳华 乔松林 李学伍 赵光辉 张改平 |
| 作者单位： | 1. 河南农业大学,河南,郑州,450002;河南省动物免疫学重点实验室,河南,郑州,450002 2. 河南农业大学,河南,郑州,450002;河南省动物免疫学重点实验室,河南,郑州,450002;河南教育学院,河南,郑州,450003 3. 河南省动物免疫学重点实验室,河南,郑州,450002 |
| 摘 要： | 为获得鸡λ轻链基因的表达蛋白,采用聚合酶链反应从鸡法氏囊B细胞cDNA文库中扩增出分泌表达蛋白的编码基因;用限制性内切酶消化后,插入克隆载体pUC19中。经PCR鉴定与序列测定证实后,以亚克隆法构建于原核表达载体PET43a的相应酶切位点。经过基因序列测定、BamHⅠ和HindⅢ双酶切鉴定证实克隆载体正确插入载体PET43a。转化宿主菌BL21(DE3),经IPTG诱导、SDS-PAGE分析表明目的蛋白得到表达;且能与His-Taq单抗相结合。 |
| 关 键 词： | 传染性法氏囊 基因 蛋白 表达 |
| 文章编号： | 1004-3268(2007)03-0109-03 |
| 修稿时间： | 2006-11-02 |
Expression of Protein Encoded by λ Light Chain Gene of Bursal B Lymphocytes in E.coli |
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| SONG Hai-tao,ZHANG Hong,WANG Li,DUAN Yan-hua,QIAO Song-lin,LI Xue-wu,ZHAO Guang-hui,ZHANG Gai-ping.Expression of Protein Encoded by λ Light Chain Gene of Bursal B Lymphocytes in E.coli[J].Journal of Henan Agricultural Sciences,2007(3):109-111. | |
| Authors: | SONG Hai-tao ZHANG Hong WANG Li DUAN Yan-hua QIAO Song-lin LI Xue-wu ZHAO Guang-hui ZHANG Gai-ping |
| Institution: | 1. Henan Agricultural University, Zhengzhou 450002 ,China; 2. Henan Key Laboratory of Animal Immunology, Zhengzhou 450002 ,China; 3. Henan Institute of Education,Zhengzhou 450003 ,China |
| Abstract: | λ light chain gene encoding the expression protein was amplified by polymerase chain reaction(PCR) from infectious bursal B lymphocyte cDNA library, and inserted into the corresponding sites of vector pUC19 after digested by restriction endonuclease to construct protocaryon expression vector PET43a.Both nucleotide sequencing and BamHⅠ and HindⅢ digestion confirmed that λ light chain gene fragment encoding the target protein was correctly inserted into the vector.The recombinant plasmid was transformed into E.coli BL21(DE3) and induction by IPTG and SDS-PAGE indicated that the target protein was expressed. |
| Keywords: | Infectious bursal disease Gene Protein Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |